BRENDA - Enzyme Database show
show all sequences of 1.14.14.93

Molecular characterization and functional expression of dihydroxypterocarpan 6a-hydroxylase, an enzyme specific for pterocarpanoid phytoalexin biosynthesis in soybean (Glycine max L.)

Schopfer, C.R.; Kochs, G.; Lottspeich, F.; Ebel, J.; FEBS Lett. 432, 182-186 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
CYP93A1 cDNA isolated from elicitor-induced soybean cells encodes the enzyme, CYP93A1 expressed in Saccharomyces cerevisiae
Glycine max
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
(6aR,11aR)-3,9-Dihydroxypterocarpan
recombinant enzyme expressed in yeast
Glycine max
0.00016
-
(6aR,11aR)-3,9-Dihydroxypterocarpan
enzyme from elicitor-treated soybean cells
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
Glycine max
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
Glycine max
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycine max
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
-
390062
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390062
Glycine max
?
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
390062
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390062
Glycine max
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
recombinant enzyme and soybean microsomes
Glycine max
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
recombinant enzyme and soybean microsomes
Glycine max
Cloned(Commentary) (protein specific)
Commentary
Organism
CYP93A1 cDNA isolated from elicitor-induced soybean cells encodes the enzyme, CYP93A1 expressed in Saccharomyces cerevisiae
Glycine max
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
(6aR,11aR)-3,9-Dihydroxypterocarpan
recombinant enzyme expressed in yeast
Glycine max
0.00016
-
(6aR,11aR)-3,9-Dihydroxypterocarpan
enzyme from elicitor-treated soybean cells
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
Glycine max
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
Glycine max
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
-
390062
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390062
Glycine max
?
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean
390062
Glycine max
(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O
-
390062
Glycine max
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
recombinant enzyme and soybean microsomes
Glycine max
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
recombinant enzyme and soybean microsomes
Glycine max
Other publictions for EC 1.14.14.93
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390062
Schopfer
Molecular characterization and ...
Glycine max
FEBS Lett.
432
182-186
1998
-
-
1
-
-
-
-
2
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
348370
Kochs
Further characterization of cy ...
Glycine max
Arch. Biochem. Biophys.
293
187-194
1992
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390061
Benveniste
Multiple forms of NADPH-cytoch ...
Glycine max
Biochem. Biophys. Res. Commun.
177
105-112
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390058
Kochs
Phytoalexin synthesis in soybe ...
Glycine max
Arch. Biochem. Biophys.
273
543-553
1989
-
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390060
Bonhoff
Race: Cultivar-specific induct ...
Glycine max
Arch. Biochem. Biophys.
246
149-154
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390057
Hagmann
Induction of phytoalexin synth ...
Glycine max
Eur. J. Biochem.
142
127-131
1984
-
-
-
-
-
-
1
-
1
1
-
1
-
2
-
-
-
-
-
2
-
1
2
-
1
1
1
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
2
-
1
2
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-