BRENDA - Enzyme Database show
show all sequences of 1.14.14.94

Leukotriene B4 omega-hydroxylase in human polymorphonuclear leukocytes. Suicidal inactivation by acetylenic fatty acids

Shak, S.; Reich, N.O.; Goldstein, I.M.; Ortiz de Montellano, P.R.; J. Biol. Chem. 260, 13023-13028 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Acetylenic fatty acids
suicidal inactivation
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
inactivation of leukotriene B4, a mediator of inflammation
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leukocyte
polymorphonuclear
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2
inactivation of leukotriene B4, a mediator of inflammation
390066
Homo sapiens
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
390066
Homo sapiens
20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Acetylenic fatty acids
suicidal inactivation
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
inactivation of leukotriene B4, a mediator of inflammation
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leukocyte
polymorphonuclear
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2
inactivation of leukotriene B4, a mediator of inflammation
390066
Homo sapiens
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
390066
Homo sapiens
20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.94
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741555
Johnson
Cytochrome P450 omega-hydroxy ...
Homo sapiens
Adv. Pharmacol.
74
223-262
2015
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1
1
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712833
Bardowell
Common variants of cytochrome ...
Homo sapiens
J. Nutr.
140
1901-1906
2010
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1
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2
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1
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1
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2
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1
1
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1
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1
1
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-
701913
Du
20-Hydroxylation is the CYP-de ...
Homo sapiens, Mus musculus, Mus musculus CD-1
Arch. Biochem. Biophys.
484
80-86
2009
1
-
1
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1
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1
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9
-
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1
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2
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2
-
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703413
Zhao
Induction of CYP4F3 by benzene ...
Homo sapiens
Drug Metab. Dispos.
37
282-291
2009
3
1
-
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-
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2
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5
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1
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1
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5
-
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1
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-
-
-
-
-
-
1
1
-
-
-
684731
Hirani
Expression of CYP4F2 in human ...
Homo sapiens
Arch. Biochem. Biophys.
478
59-68
2008
-
-
1
-
-
-
-
-
1
-
-
1
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3
-
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1
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5
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5
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1
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1
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1
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703013
Mao
Differential modulation of leu ...
Homo sapiens
Cancer Prev. Res. (Phila)
1
266-274
2008
1
-
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-
1
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-
-
3
-
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1
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1
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1
1
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-
685340
Kikuta
Expression and physiological f ...
Homo sapiens
Biochim. Biophys. Acta
1771
1439-1445
2007
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-
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-
-
2
-
1
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1
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3
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6
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6
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1
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1
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1
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2
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1
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1
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6
-
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6
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1
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1
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-
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689399
Stec
Functional polymorphism in hum ...
Homo sapiens
Physiol. Genomics
30
74-81
2007
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1
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3
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1
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2
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1
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3
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1
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1
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3
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674761
Christmas
Cytochrome P-450 4F18 is the l ...
Mus musculus
J. Biol. Chem.
281
7189-7196
2006
1
-
1
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1
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2
-
4
-
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6
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6
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3
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657762
Mizukami
Induction of cytochrome CYP4F3 ...
Homo sapiens
Biochem. Biophys. Res. Commun.
314
104-109
2004
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1
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1
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659668
le Quere
Human CYP4F3s are the main cat ...
Homo sapiens
J. Lipid Res.
45
1446-1458
2004
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9
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2
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5
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658724
Kalsotra
Inflammatory prompts produce i ...
Rattus norvegicus
FEBS Lett.
555
236-242
2003
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2
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1
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658922
Kikuta
-
Catalytic properties of two hu ...
Homo sapiens
Int. Congr. Ser.
1233
139-142
2002
-
-
1
-
-
-
-
12
-
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1
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1
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2
8
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1
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12
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1
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2
8
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660409
Kikuta
Prostaglandin and leukotriene ...
Homo sapiens
Prostaglandins
68-69
345-362
2002
-
-
1
-
-
-
-
1
1
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1
3
-
6
-
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1
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10
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2
21
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1
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1
1
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1
3
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1
-
10
-
2
21
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390075
Kikuta
Characterization of human live ...
Homo sapiens
J. Biochem.
127
1047-1052
2000
-
-
1
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-
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1
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1
1
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1
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1
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1
3
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1
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3
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390076
Kikuta
Expression and catalytic activ ...
Mus musculus
Arch. Biochem. Biophys.
383
225-232
2000
-
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1
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1
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1
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4
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390071
Christmas
Expression of the CYP4F3 gene. ...
Homo sapiens
J. Biol. Chem.
274
21191-21199
1999
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1
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2
1
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1
1
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2
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2
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1
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2
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390074
Kikuta
Expression and molecular cloni ...
Homo sapiens
DNA Cell Biol.
18
723-730
1999
-
-
1
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1
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2
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5
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2
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1
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5
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2
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390073
Kikuta
Purification and characterizat ...
Homo sapiens
Arch. Biochem. Biophys.
355
201-205
1998
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1
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2
2
1
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1
1
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4
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1
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1
1
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3
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1
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1
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2
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2
1
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1
1
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1
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1
1
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3
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1
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390072
Ikehata
5-Aminosalicylic acid inhibits ...
Homo sapiens
Prostaglandins Leukot. Essent. Fatty Acids
50
317-320
1994
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1
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1
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2
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390070
Sumimoto
Leukotriene B4 omega-hydroxyla ...
Rattus norvegicus
J. Biochem.
108
215-221
1990
-
-
-
-
-
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3
2
1
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1
-
3
-
-
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-
-
2
-
-
3
-
-
-
-
-
1
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1
2
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1
-
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3
2
2
1
-
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1
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2
-
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3
-
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-
1
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390068
Soberman
Leukotriene B4 20-hydroxylase ...
Homo sapiens
Methods Enzymol.
163
349-353
1988
-
-
-
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1
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1
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2
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2
1
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2
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1
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1
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1
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1
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1
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1
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2
1
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2
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1
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1
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-
-
390069
Sumimoto
Characterization of human neut ...
Homo sapiens
Eur. J. Biochem.
172
315-324
1988
-
-
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2
2
1
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1
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3
-
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1
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2
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1
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1
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2
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390063
Romano
Biochemical characterization o ...
Rattus norvegicus
J. Biol. Chem.
262
1590-1595
1987
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2
3
2
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4
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2
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390067
Soberman
Stereochemical requirements fo ...
Homo sapiens
J. Biol. Chem.
262
12421-12427
1987
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2
4
1
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6
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6
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390064
Soberman
Identification and functional ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
82
2292-2295
1985
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2
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2
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1
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1
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390066
Shak
Leukotriene B4 omega-hydroxyla ...
Homo sapiens
J. Biol. Chem.
260
13023-13028
1985
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1
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2
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390065
Powell
Properties of leukotriene B4 2 ...
Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
259
3082-3089
1984
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