BRENDA - Enzyme Database show
show all sequences of 1.14.14.96

Chlorogenic acid biosynthesis: Characterization of a light-induced microsomal 5-O-(4-coumaroyl)-D-quinate/shikimate 3'-hydroxylase from carrot (Daucus carota L.) cell suspension cultures

Kuhnl, T.; Koch, U.; Heller, W.; Wellmann, E.; Arch. Biochem. Biophys. 258, 226-232 (1987)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
diethyldithiocarbonate
2fold activation at 0.2-2 mM
Daucus carota
KCN
2fold activation at 1-10 mM
Daucus carota
light exposure
4fold activation after 20 h irradiation at 350 nm
Daucus carota
Inhibitors
Inhibitors
Commentary
Organism
Structure
cytochrome c
76% inhibition at 0.1 mM
Daucus carota
diethyldicarbonate
31% inhibition at 2 mM
Daucus carota
p-chloromercuribenzoate
32% inhibition at 0.5 mM
Daucus carota
Tetcyclacis
38% inhibition at 0.01 mM
Daucus carota
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Daucus carota
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2
Daucus carota
specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light
trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Daucus carota
?
trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2
Daucus carota
70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer
trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Daucus carota
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Daucus carota
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Daucus carota
-
Storage Stability
Storage Stability
Organism
-70°C, stable for several months
Daucus carota
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2
specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light
395989
Daucus carota
trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
395989
Daucus carota
?
trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2
70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer
395989
Daucus carota
trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O
-
395989
Daucus carota
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
half-life: 10 min
Daucus carota
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Daucus carota
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.3
-
half-maximal activity
Daucus carota
8.6
-
half-maximal activity
Daucus carota
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
diethyldithiocarbonate
2fold activation at 0.2-2 mM
Daucus carota
KCN
2fold activation at 1-10 mM
Daucus carota
light exposure
4fold activation after 20 h irradiation at 350 nm
Daucus carota
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cytochrome c
76% inhibition at 0.1 mM
Daucus carota
diethyldicarbonate
31% inhibition at 2 mM
Daucus carota
p-chloromercuribenzoate
32% inhibition at 0.5 mM
Daucus carota
Tetcyclacis
38% inhibition at 0.01 mM
Daucus carota
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Daucus carota
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2
Daucus carota
specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light
trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Daucus carota
?
trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2
Daucus carota
70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer
trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Daucus carota
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Daucus carota
-
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, stable for several months
Daucus carota
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2
specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light
395989
Daucus carota
trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
395989
Daucus carota
?
trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2
70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer
395989
Daucus carota
trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O
-
395989
Daucus carota
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
half-life: 10 min
Daucus carota
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Daucus carota
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.3
-
half-maximal activity
Daucus carota
8.6
-
half-maximal activity
Daucus carota
Other publictions for EC 1.14.14.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742154
Sykes
Down-regulation of p-coumaroy ...
Eucalyptus urophylla x Eucalyptus grandis
Biotechnol. Biofuels
8
128
2015
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395989
Kuhnl
Chlorogenic acid biosynthesis: ...
Daucus carota
Arch. Biochem. Biophys.
258
226-232
1987
3
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