Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diethyldithiocarbonate | 2fold activation at 0.2-2 mM | Daucus carota | |
KCN | 2fold activation at 1-10 mM | Daucus carota | |
light exposure | 4fold activation after 20 h irradiation at 350 nm | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | 76% inhibition at 0.1 mM | Daucus carota | |
diethyldicarbonate | 31% inhibition at 2 mM | Daucus carota | |
p-chloromercuribenzoate | 32% inhibition at 0.5 mM | Daucus carota | |
Tetcyclacis | 38% inhibition at 0.01 mM | Daucus carota |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Daucus carota | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2 | Daucus carota | specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light | trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2 | Daucus carota | 70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer | trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Daucus carota | - |
Storage Stability | Organism |
---|---|
-70°C, stable for several months | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2 | specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light | Daucus carota | trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2 | 70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer | Daucus carota | trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life: 10 min | Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Daucus carota |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
half-maximal activity | Daucus carota |
8.6 | - |
half-maximal activity | Daucus carota |