BRENDA - Enzyme Database
show all sequences of 1.14.14.96

Down-regulation of p-coumaroyl quinate/shikimate 3-hydroxylase (C3H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release

Sykes, R.W.; Gjersing, E.L.; Foutz, K.; Rottmann, W.H.; Kuhn, S.A.; Foster, C.E.; Ziebell, A.; Turner, G.B.; Decker, S.R.; Hinchee, M.A.; Davis, M.F.; Biotechnol. Biofuels 8, 128 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
biofuel production
lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. Eucalyptus trees with down-regulated cinnamate 4-hydroxylase (C4H) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) expression display lowered overall lignin content. Lowering lignin content rather than altering sinapyl alcohol/coniferyl alcohol/4-coumaryl alcohol ratios is found to have the largest impact on reducing recalcitrance of the transgenic eucalyptus variants. The development of lower recalcitrance trees opens up the possibility of using alternative pretreatment strategies in biomass conversion processes that can reduce processing costs
Eucalyptus urophylla x Eucalyptus grandis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Eucalyptus urophylla x Eucalyptus grandis
-
-
-
Application (protein specific)
Application
Commentary
Organism
biofuel production
lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. Eucalyptus trees with down-regulated cinnamate 4-hydroxylase (C4H) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) expression display lowered overall lignin content. Lowering lignin content rather than altering sinapyl alcohol/coniferyl alcohol/4-coumaryl alcohol ratios is found to have the largest impact on reducing recalcitrance of the transgenic eucalyptus variants. The development of lower recalcitrance trees opens up the possibility of using alternative pretreatment strategies in biomass conversion processes that can reduce processing costs
Eucalyptus urophylla x Eucalyptus grandis
General Information
General Information
Commentary
Organism
malfunction
Eucalyptus trees with down-regulated p-coumaroyl quinate/shikimate 3'-hydroxylase expression display lowered overall lignin content
Eucalyptus urophylla x Eucalyptus grandis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
Eucalyptus trees with down-regulated p-coumaroyl quinate/shikimate 3'-hydroxylase expression display lowered overall lignin content
Eucalyptus urophylla x Eucalyptus grandis
Other publictions for EC 1.14.14.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742154
Sykes
Down-regulation of p-coumaroy ...
Eucalyptus urophylla x Eucalyptus grandis
Biotechnol. Biofuels
8
128
2015
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395989
Kuhnl
Chlorogenic acid biosynthesis: ...
Daucus carota
Arch. Biochem. Biophys.
258
226-232
1987
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