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Literature summary for 1.14.14.97 extracted from

  • Rueffer, M.; Zenk, M.H.
    Enzymatic formation of protopines by a microsomal cytochrome P-450 system of Corydalis vaginans (1987), Tetrahedron Lett., 28, 5307-5310.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ancymidol
-
Corydalis vaginans
CO reversible by light Corydalis vaginans
cytochrome c
-
Corydalis vaginans
ketoconazole
-
Corydalis vaginans
Metyrapone
-
Corydalis vaginans
Prochloraz
-
Corydalis vaginans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
cis-N-methylcanadine
-
Corydalis vaginans
0.0625
-
NADPH
-
Corydalis vaginans

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome membrane bound Corydalis vaginans
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 Corydalis vaginans (R)-cis/trans-N-methyl-canadine is no substrate allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 Corydalis vaginans hydroxylation in benzylisoquinoline metabolism allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Corydalis vaginans
-
papaveraceae
-

Reaction

Reaction Comment Organism Reaction ID
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 = allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O stereo- and regiospecificity Corydalis vaginans

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Corydalis vaginans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0255
-
-
Corydalis vaginans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 no activity without molecular oxygen Corydalis vaginans allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 (R)-cis/trans-N-methyl-canadine is no substrate Corydalis vaginans allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation in benzylisoquinoline metabolism Corydalis vaginans allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methylcorydalmine + [reduced NADPH-hemoprotein reductase] + O2
-
Corydalis vaginans muramine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methylstylopine + [reduced NADPH-hemoprotein reductase] + O2
-
Corydalis vaginans protopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methyltetrahydropalmitine + [reduced NADPH-hemoprotein reductase] + O2
-
Corydalis vaginans muramine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-cis-N-methyltetrahydrothalifendine + [reduced NADPH-hemoprotein reductase] + O2
-
Corydalis vaginans thalictrisine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-N-methylcanadine + NADPH + H+ + O2
-
Corydalis vaginans allocryptopine + NADP+ + H2O
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Corydalis vaginans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 45 half-maximal activity at 10°C and 45°C Corydalis vaginans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Corydalis vaginans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.7 half-maximal activity at pH 6.5 and pH 9.7 Corydalis vaginans

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Corydalis vaginans