BRENDA - Enzyme Database show
show all sequences of 1.14.14.97

Enzymatic formation of protopines by a microsomal cytochrome P-450 system of Corydalis vaginans

Rueffer, M.; Zenk, M.H.; Tetrahedron Lett. 28, 5307-5310 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidol
-
Corydalis vaginans
CO
reversible by light
Corydalis vaginans
cytochrome c
-
Corydalis vaginans
ketoconazole
-
Corydalis vaginans
Metyrapone
-
Corydalis vaginans
Prochloraz
-
Corydalis vaginans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0125
-
cis-N-methylcanadine
-
Corydalis vaginans
0.0625
-
NADPH
-
Corydalis vaginans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
membrane bound
Corydalis vaginans
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Corydalis vaginans
(R)-cis/trans-N-methyl-canadine is no substrate
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Corydalis vaginans
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Corydalis vaginans
hydroxylation in benzylisoquinoline metabolism
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Corydalis vaginans
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corydalis vaginans
-
papaveraceae
-
Reaction
Reaction
Commentary
Organism
(S)-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 = allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
stereo- and regiospecificity
Corydalis vaginans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Corydalis vaginans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0255
-
-
Corydalis vaginans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
no activity without molecular oxygen
285317
Corydalis vaginans
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(R)-cis/trans-N-methyl-canadine is no substrate
285317
Corydalis vaginans
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation in benzylisoquinoline metabolism
285317
Corydalis vaginans
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methylcorydalmine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
muramine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-cis-N-methylstylopine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
protopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methyltetrahydropalmitine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
muramine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-cis-N-methyltetrahydrothalifendine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
thalictrisine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Corydalis vaginans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
45
half-maximal activity at 10°C and 45°C
Corydalis vaginans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Corydalis vaginans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.7
half-maximal activity at pH 6.5 and pH 9.7
Corydalis vaginans
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Corydalis vaginans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Corydalis vaginans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidol
-
Corydalis vaginans
CO
reversible by light
Corydalis vaginans
cytochrome c
-
Corydalis vaginans
ketoconazole
-
Corydalis vaginans
Metyrapone
-
Corydalis vaginans
Prochloraz
-
Corydalis vaginans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0125
-
cis-N-methylcanadine
-
Corydalis vaginans
0.0625
-
NADPH
-
Corydalis vaginans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
membrane bound
Corydalis vaginans
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Corydalis vaginans
(R)-cis/trans-N-methyl-canadine is no substrate
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Corydalis vaginans
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
Corydalis vaginans
hydroxylation in benzylisoquinoline metabolism
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Corydalis vaginans
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Corydalis vaginans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0255
-
-
Corydalis vaginans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
no activity without molecular oxygen
285317
Corydalis vaginans
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
(R)-cis/trans-N-methyl-canadine is no substrate
285317
Corydalis vaginans
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation in benzylisoquinoline metabolism
285317
Corydalis vaginans
allocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methylcorydalmine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
muramine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-cis-N-methylstylopine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
protopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285317
Corydalis vaginans
?
(S)-cis-N-methyltetrahydropalmitine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
muramine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-cis-N-methyltetrahydrothalifendine + [reduced NADPH-hemoprotein reductase] + O2
-
285317
Corydalis vaginans
thalictrisine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Corydalis vaginans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
45
half-maximal activity at 10°C and 45°C
Corydalis vaginans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Corydalis vaginans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.7
half-maximal activity at pH 6.5 and pH 9.7
Corydalis vaginans
Other publictions for EC 1.14.14.97
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285317
Rueffer
-
Enzymatic formation of protopi ...
Corydalis vaginans
Tetrahedron Lett.
28
5307-5310
1987
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-
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-
-
-
6
2
1
-
-
2
-
1
-
-
-
1
-
1
1
-
7
-
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
2
1
-
-
2
-
-
-
-
-
1
1
-
7
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-