BRENDA - Enzyme Database
show all sequences of 1.14.14.98

Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells

Takemura, T.; Ikezawa, N.; Iwasa, K.; Sato, F.; Phytochemistry 91, 100-108 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Eschscholzia californica
Inhibitors
Inhibitors
Commentary
Organism
Structure
ketoconazole
less than 2% activity at 0.025 mM
Eschscholzia californica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00645
-
6-hydroxyprotopine
in 50 mM HEPES/NaOH (pH 7.6), at 30°C
Eschscholzia californica
0.0087
-
6-hydroxyallocryptopine
in 50 mM HEPES/NaOH (pH 7.6), at 30°C
Eschscholzia californica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Eschscholzia californica
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Eschscholzia californica
F2Z9C1
california poppy
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
13-oxoprotopine + [reduced NADPH-hemoprotein reductase] + O2
-
728446
Eschscholzia californica
?
-
-
-
?
allocryptopine + [reduced NADPH-hemoprotein reductase] + O2
-
728446
Eschscholzia californica
6-hydroxyallocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
corycavine + [reduced NADPH-hemoprotein reductase] + O2
-
728446
Eschscholzia californica
corynoloxine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with protopine-N-oxide, reticuline, scoulerine, and N-methyl stylopine
728446
Eschscholzia californica
?
-
-
-
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
i.e. dihydrosanguinarine
728446
Eschscholzia californica
6-hydroxyprotopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Eschscholzia californica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ketoconazole
less than 2% activity at 0.025 mM
Eschscholzia californica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00645
-
6-hydroxyprotopine
in 50 mM HEPES/NaOH (pH 7.6), at 30°C
Eschscholzia californica
0.0087
-
6-hydroxyallocryptopine
in 50 mM HEPES/NaOH (pH 7.6), at 30°C
Eschscholzia californica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Eschscholzia californica
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
13-oxoprotopine + [reduced NADPH-hemoprotein reductase] + O2
-
728446
Eschscholzia californica
?
-
-
-
?
allocryptopine + [reduced NADPH-hemoprotein reductase] + O2
-
728446
Eschscholzia californica
6-hydroxyallocryptopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
corycavine + [reduced NADPH-hemoprotein reductase] + O2
-
728446
Eschscholzia californica
corynoloxine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with protopine-N-oxide, reticuline, scoulerine, and N-methyl stylopine
728446
Eschscholzia californica
?
-
-
-
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
i.e. dihydrosanguinarine
728446
Eschscholzia californica
6-hydroxyprotopine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.98
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746409
Verma
Improved sanguinarine product ...
Papaver somniferum
Protoplasma
251
1359-1371
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
726878
Beaudoin
Isolation and characterization ...
Papaver somniferum
Biochem. Biophys. Res. Commun.
431
597-603
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728446
Takemura
Molecular cloning and characte ...
Eschscholzia californica
Phytochemistry
91
100-108
2013
-
-
1
-
-
-
1
2
1
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7391
Tanahashi
-
Elicitor induction and charact ...
Chelidonium majus, Dicentra cucullaria, Eschscholzia californica, Eschscholzia lobbii, Fumaria parviflora, no activity in Berberis stolonifera, no activity in Catharanthus roseus
Phytochemistry
29
1113-1122
1990
-
-
-
-
-
-
5
1
5
-
-
5
-
7
-
-
-
-
-
5
-
-
10
-
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
1
5
-
-
5
-
-
-
-
-
5
-
-
10
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-