BRENDA - Enzyme Database show
show all sequences of 1.14.14.99

A candidate cDNA clone for (-)-limonene-7-hydroxylase from Perilla frutescens

Mau, C.J.; Karp, F.; Ito, M.; Honda, G.; Croteau, R.B.; Phytochemistry 71, 373-379 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of the chimeric protein in Escherichia coli
Perilla frutescens
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of a chimeric mutant enzyme by fusion of the N-terminal membrane insertion domain of the limonene-3-hydroxylase into limonene 7-hydroxylase. The kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives with a distribution of 33%, 14% and 53%, respectively
Perilla frutescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
Perilla frutescens
-
(-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Perilla frutescens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
713236
Perilla frutescens
(-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
the kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives, i.e. (-)-perillyl alcohol, (-)-trans-isopiperitenol, and (-)-trans-carveol, with a distribution of 33%, 14% and 53%, respectively, thus it performs the reaction of EC 1.14.13.47 and 1.14.13.48, overview
713236
Perilla frutescens
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
recombinant chimeric mutant enzyme
Perilla frutescens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.75
8.5
half-maximal activity at pH 6.75 and 8.5, recombinant chimeric mutant enzyme
Perilla frutescens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of the chimeric protein in Escherichia coli
Perilla frutescens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of a chimeric mutant enzyme by fusion of the N-terminal membrane insertion domain of the limonene-3-hydroxylase into limonene 7-hydroxylase. The kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives with a distribution of 33%, 14% and 53%, respectively
Perilla frutescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
Perilla frutescens
-
(-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
713236
Perilla frutescens
(-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
the kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives, i.e. (-)-perillyl alcohol, (-)-trans-isopiperitenol, and (-)-trans-carveol, with a distribution of 33%, 14% and 53%, respectively, thus it performs the reaction of EC 1.14.13.47 and 1.14.13.48, overview
713236
Perilla frutescens
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
recombinant chimeric mutant enzyme
Perilla frutescens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.75
8.5
half-maximal activity at pH 6.75 and 8.5, recombinant chimeric mutant enzyme
Perilla frutescens
Other publictions for EC 1.14.14.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745019
Wriessnegger
Enhancing cytochrome P450-med ...
Mentha x piperita
Fungal Genet. Biol.
89
114-125
2016
1
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712853
Dolzhenko
UV-B modulates the interplay b ...
Mentha x piperita
J. Photochem. Photobiol. B Biol.
100
67-75
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
713236
Mau
A candidate cDNA clone for (-) ...
Perilla frutescens
Phytochemistry
71
373-379
2010
-
-
1
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
660103
Mahmoud
Cosuppression of limonene-3-hy ...
Mentha x piperita
Phytochemistry
65
547-554
2004
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660093
Bertea
Molecular evaluation of a spea ...
Mentha x gracilis
Phytochemistry
64
1203-1211
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390089
Wust
Hydroxylation of limonene enan ...
Mentha x piperita
Arch. Biochem. Biophys.
387
125-136
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390086
Schalk
A single amino acid substituti ...
Mentha spicata
Proc. Natl. Acad. Sci. USA
97
11948-11953
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390088
Haudenschild
Functional expression of regio ...
Mentha x piperita
Arch. Biochem. Biophys.
379
127-136
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390087
Lupien
Regiospecific cytochrome P450 ...
Mentha x piperita
Arch. Biochem. Biophys.
368
181-192
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390085
Gershenzon
Isolation of secretory cells f ...
Mentha x piperita
Anal. Biochem.
200
130-138
1992
-
-
-
-
-
3
-
-
1
-
-
-
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390084
Croteau
Biochemical characterization o ...
Mentha x gracilis, Mentha x piperita
Plant Physiol.
96
744-752
1991
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390083
Karp
Monoterpene biosynthesis: spec ...
Mentha x piperita
Arch. Biochem. Biophys.
276
219-226
1990
2
-
-
-
-
-
9
1
1
-
-
1
-
1
-
-
-
1
-
1
1
-
6
-
1
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
9
-
1
1
-
-
1
-
-
-
-
-
1
1
-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-