Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.B11 extracted from

  • Siminszky, B.; Gavilano, L.; Bowen, S.W.; Dewey, R.E.
    Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase (2005), Proc. Natl. Acad. Sci. USA, 102, 14919-14924.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.B11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotine + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum
-
 nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP82E4v1 is responsible for the conversion of nicotine to nornicotine ?
nicotine + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum nornicotine is a secondary tobacco alkaloid that is produced by the N-demethylation of nicotine. Nornicotine production and accumulation in tobacco are undesirable because nornicotine serves as the precursor in the synthesis of the carcinogen N-nitrosonornicotine during the curing and processing of tobacco nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q38Q86 CYP82E4
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf cured leaf Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotine + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP82E4v1 is responsible for the conversion of nicotine to nornicotine ?
nicotine + [reduced NADPH-hemoprotein reductase] + O2 nornicotine is a secondary tobacco alkaloid that is produced by the N-demethylation of nicotine. Nornicotine production and accumulation in tobacco are undesirable because nornicotine serves as the precursor in the synthesis of the carcinogen N-nitrosonornicotine during the curing and processing of tobacco Nicotiana tabacum nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Nicotiana tabacum