BRENDA - Enzyme Database show
show all sequences of 1.14.14.B11

Molecular dynamics analysis reveals structural insights into mechanism of nicotine N-demethylation catalyzed by tobacco cytochrome P450 mono-oxygenase

Wang, S.; Yang, S.; An, B.; Wang, S.; Yin, Y.; Lu, Y.; Xu, Y.; Hao, D.; PLoS ONE 6, e23342 (2011)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C330W
inactive
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2
-
728555
Nicotiana tabacum
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Nicotiana tabacum
NADPH
-
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Nicotiana tabacum
NADPH
-
Nicotiana tabacum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C330W
inactive
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2
-
728555
Nicotiana tabacum
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.B11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746003
Liedschulte
Identification of CYP82E21 as ...
Nicotiana tabacum
Phytochemistry
131
9-16
2016
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-
1
-
1
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-
-
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1
-
4
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4
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1
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2
-
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1
2
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1
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1
-
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-
-
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4
-
-
1
-
-
-
-
-
-
-
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-
3
3
-
-
-
746124
Zhao
RNA interference of the nicot ...
Nicotiana tabacum
Plant Physiol. Biochem.
107
214-221
2016
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-
1
-
1
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2
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4
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3
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2
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2
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1
2
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1
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3
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2
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1
1
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726648
Lian
-
Data mining of nicotine demeth ...
Nicotiana tabacum
Adv. Mater. Res.
787
356-360
2013
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1
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1
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1
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-
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727654
Cai
Variable nornicotine enantiome ...
Nicotiana tabacum
J. Agric. Food Chem.
60
11586-11591
2012
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-
-
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-
-
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-
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1
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2
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-
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2
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1
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1
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1
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1
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2
-
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1
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727935
Cai
Enantioselective demethylation ...
Nicotiana tabacum
J. Biol. Chem.
287
42804-42811
2012
-
-
-
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2
-
3
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1
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2
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2
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2
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2
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1
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2
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-
728461
Pakdeechanuan
Non-functionalization of two C ...
Nicotiana alata, Nicotiana langsdorffii
Plant Cell Physiol.
53
2038-2046
2012
-
-
2
-
-
-
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5
-
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5
-
9
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4
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5
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4
-
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5
10
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5
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5
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10
-
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5
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728555
Wang
Molecular dynamics analysis re ...
Nicotiana tabacum
PLoS ONE
6
e23342
2011
-
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1
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1
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2
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1
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1
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2
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2
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1
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1
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1
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1
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689456
Lewis
RNA interference (RNAi)-induce ...
Nicotiana tabacum
Plant Biotechnol. J.
6
346-354
2008
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1
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1
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4
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1
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1
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1
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687594
Gavilano
Functional analysis of nicotin ...
Nicotiana tabacum, Nicotiana tomentosiformis
J. Biol. Chem.
282
249-256
2007
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-
-
6
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-
2
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6
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1
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2
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11
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2
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2
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2
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-
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-
689241
Chakrabarti
Inactivation of the cytochrome ...
Nicotiana sylvestris
New Phytol.
175
565-574
2007
-
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1
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3
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1
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5
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1
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1
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1
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3
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1
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1
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1
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689487
Gavilano
Isolation and characterization ...
Nicotiana tabacum
Plant Cell Physiol.
48
1567-1574
2007
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1
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1
1
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5
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1
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1
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700634
Xu
Biochemical and molecular char ...
Nicotiana tabacum
Physiol. Plant.
129
307-319
2007
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1
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7
1
2
1
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1
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1
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1
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3
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2
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1
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7
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1
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1
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1
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1
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3
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1
1
1
1
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687223
Gavilano
Genetic engineering of Nicotia ...
Nicotiana tabacum
J. Agric. Food Chem.
54
9071-9078
2006
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2
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1
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3
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676779
Siminszky
Conversion of nicotine to norn ...
Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
102
14919-14924
2005
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1
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1
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1
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1
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686348
Yamanaka
CYP2A6 AND CYP2B6 are involved ...
Homo sapiens
Drug Metab. Dispos.
33
1811-1818
2005
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1
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3
2
1
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3
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1
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1
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1
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688634
Hao
-
Evidence in favor of an oxidat ...
Nicotiana tabacum
J. Plant Physiol.
152
420-426
1998
1
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