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Literature summary for 1.14.14.B11 extracted from

  • Liedschulte, V.; Schwaar, J.D.; Laparra, H.; Vuarnoz, A.; Philippon, B.; Bakaher, N.; Sierro, N.; Bovet, L.; Lang, G.; Goepfert, S.
    Identification of CYP82E21 as a functional nicotine N-demethylase in tobacco flowers (2016), Phytochemistry, 131, 9-16 .
    View publication on PubMed
Search for more literature for 1.14.14.B11

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP82E21, unrooted phylogenetic analysis and tree of CYP82E21 and related CYP82E coding sequences, quantitative PCR enzyme expression analysis, ectopic expression of the coding sequence in a tobacco line lacking functional CYP82E4, CYP82E5 and CYP82E10 genes, resulting in an eightfold increase of nicotine demethylation compared to the control plants confirming the nicotine N-demethylase activity of CYP82E21 Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information enzyme silencing by introducing a CYP82E21-specific RNAi construct in ovaries, silencing of CYP82E21 leads to up to 90% reduced nicotine conversion in flower ovaries Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotine + NADPH + H+ Nicotiana tabacum
-
 nornicotine + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 var. TN90
-

Source Tissue

Source Tissue Comment Organism Textmining
flower regarding sepals, petals, ovaries, stigma including style, filaments and anthers, CYP82E21 expression is found almost exclusively in ovaries, with very low expression levels observed in other flower organs Nicotiana tabacum
-
additional information CYP82E21 expression pattern analysis, CYP82E21 is specifically expressed in ovaries but not expressed in leaves Nicotiana tabacum
-
ovary specific for Nicotiana tabacum
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plant ovary specific for Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotine + NADPH + H+
-
 Nicotiana tabacum nornicotine + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
CYP82E21
-
 Nicotiana tabacum
nicotine N-demethylase
-
 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Nicotiana tabacum
NADPH
-
 Nicotiana tabacum

General Information

General Information Comment Organism
evolution N-demethylase enzymes (NND) belongs to the family of cytochrome P450 monooxygenases (CYP). The CYP82E21 gene is found in all Nicotiana tabacum cultivars analysed and originates from the tobacco ancestor Nicotiana tomentosiformis Nicotiana tabacum
malfunction nornicotine formation can be reduced in ovaries by introducing a CYP82E21-specific RNAi construct Nicotiana tabacum
physiological function the CYP82E21 gene encodes a functional ovary-specific nicotine N-demethylase, nornicotine is formed by oxidative demethylation of nicotine Nicotiana tabacum