BRENDA - Enzyme Database show
show all sequences of 1.14.14.B13

New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase

Humphreys, J.M.; Hemm, M.R.; Chapple, C.; Proc. Natl. Acad. Sci. USA 96, 10045-10050 (1999)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
coniferaldehyde
pH 7.8, 30°C
Arabidopsis thaliana
0.003
-
coniferyl alcohol
pH 7.8, 30°C
Arabidopsis thaliana
1
-
ferulate
pH 7.8, 30°C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q42600
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
stem
flowering plant stem
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
coniferyl alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ferulate + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyferulate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
heme-thiolate (P-450) monooxygenase
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
heme-thiolate (P-450) monooxygenase
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
coniferaldehyde
pH 7.8, 30°C
Arabidopsis thaliana
0.003
-
coniferyl alcohol
pH 7.8, 30°C
Arabidopsis thaliana
1
-
ferulate
pH 7.8, 30°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
stem
flowering plant stem
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
coniferyl alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ferulate + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyferulate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.B13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743699
Humphreys
New routes for lignin biosynt ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
96
10045-10050
1999
-
-
-
-
-
-
-
3
-
-
1
1
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743700
Meyer
Ferulate-5-hydroxylase from A ...
Arabidopsis thaliana
Proc. Natl. Acad.Sci. USA
93
6869-6874
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-