Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofuran | activation | Pseudomonas sp. |
General Stability | Organism |
---|---|
dialysis, loss of activity in crude extract of Pseudomonas sp. C5, restorable by NADPH-addition | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | activity sediments after 14 h centrifugation of crude extract at 140000 * g, not after 2 h at 100000 * g, Pseudomonas sp. C5 | Pseudomonas sp. | 5737 | - |
cytoplasm | activity sediments after 14 h centrifugation of crude extract at 140000 * g, not after 2 h at 100000 * g, Pseudomonas sp. C5 | Pseudomonas putida | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | Pseudomonas sp. | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
(+)-camphor + putidaredoxin + O2 | Pseudomonas putida | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
(+)-camphor + putidaredoxin + O2 | Pseudomonas putida C1 / ATCC 17453 | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida | - |
Accession Number PC00183 | - |
Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
Pseudomonas sp. | - |
soil isolate, strain C5 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.016 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
0-4°C, after 24-96 h, the resuspended pellet of 140000*g sedimentation of Pseudomonas sp. C5 loses hydroxylation capacity, restorable with NADPH and THF | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | - |
Pseudomonas sp. | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
(+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
(+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida C1 / ATCC 17453 | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
1,2-campholide + putidaredoxin + O2 | - |
Pseudomonas sp. | 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O | - |
? | |
1,2-campholide + putidaredoxin + O2 | - |
Pseudomonas putida | 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O | - |
? | |
1,2-campholide + putidaredoxin + O2 | - |
Pseudomonas putida C1 / ATCC 17453 | 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas sp. | |
FMN | - |
Pseudomonas putida | |
NADPH | - |
Pseudomonas putida | |
NADPH | requirement, instead of NADH, with campholide as substrate, Pseudomonas sp. C5 | Pseudomonas sp. |