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Literature summary for 1.14.15.1 extracted from

  • Hedegaard, J.; Gunsalus, I.C.
    Mixed function oxidation. IV. An induced methylene hydroxylase in camphor oxidation (1965), J. Biol. Chem., 240, 4038-4043.
    View publication on PubMed
Search for more literature for 1.14.15.1

Activating Compound

Activating Compound Comment Organism Structure
tetrahydrofuran activation Pseudomonas sp.

General Stability

General Stability Organism
dialysis, loss of activity in crude extract of Pseudomonas sp. C5, restorable by NADPH-addition Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm activity sediments after 14 h centrifugation of crude extract at 140000 * g, not after 2 h at 100000 * g, Pseudomonas sp. C5 Pseudomonas sp. 5737
-
cytoplasm activity sediments after 14 h centrifugation of crude extract at 140000 * g, not after 2 h at 100000 * g, Pseudomonas sp. C5 Pseudomonas putida 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + putidaredoxin + O2 Pseudomonas sp.
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
(+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
(+)-camphor + putidaredoxin + O2 Pseudomonas putida C1 / ATCC 17453
-
 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida
-
 Accession Number PC00183
-
Pseudomonas putida C1 / ATCC 17453
-
-
-
Pseudomonas sp.
-
 soil isolate, strain C5
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.016
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
0-4°C, after 24-96 h, the resuspended pellet of 140000*g sedimentation of Pseudomonas sp. C5 loses hydroxylation capacity, restorable with NADPH and THF Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + putidaredoxin + O2
-
 Pseudomonas sp. (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
(+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
(+)-camphor + putidaredoxin + O2
-
 Pseudomonas putida C1 / ATCC 17453 (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
1,2-campholide + putidaredoxin + O2
-
 Pseudomonas sp. 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O
-
 ?
1,2-campholide + putidaredoxin + O2
-
 Pseudomonas putida 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O
-
 ?
1,2-campholide + putidaredoxin + O2
-
 Pseudomonas putida C1 / ATCC 17453 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Pseudomonas sp.
FMN
-
 Pseudomonas putida
NADPH
-
 Pseudomonas putida
NADPH requirement, instead of NADH, with campholide as substrate, Pseudomonas sp. C5 Pseudomonas sp.