Application | Comment | Organism |
---|---|---|
synthesis | selective enzymatic oxidation of (+)-alpha-pinene to verbenol, verbenone, or myrtenol by enzyme mutants | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
mutant Y96F/F87W/V247L, binding of substrate (+)-alpha-pinene in two orientations related by rotation of the molecule | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
F87A/Y96F | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
F87L/Y96F | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
F87W/Y96F | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
F87W/Y96F/L244A | enhanced binding and oxidation of (+)-alpha-pinene, production of 86% (+)-cis-verbenol + 5% (+)-verbenone | Pseudomonas putida |
F87W/Y96F/L244A/V247L | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
F87W/Y96F/V247L | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
Y96F | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
Y96F/L244A/V247L | enhanced binding and oxidation of (+)-alpha-pinene, production of 55% (+)-cis-verbenol + 32% (+)-verbenone | Pseudomonas putida |
Y96F/V247L | enhanced binding and oxidation of (+)-alpha-pinene | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P00183 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-alpha-pinene + putidaredoxin + O2 | - |
Pseudomonas putida | (+)-cis-verbenol + (+)-myrtenol + (+)-verbenone + oxidized putidaredoxin + H2O | - |
? |