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Literature summary for 1.14.15.1 extracted from

  • Spolitak, T.; Dawson, J.H.; Ballou, D.P.
    Reaction of ferric cytochrome P450cam with peracids: kinetic characterization of intermediates on the reaction pathway (2005), J. Biol. Chem., 280, 20300-20309.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information stopped-flow kinetics at different pH and temperature Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron heme iron Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O reaction mechanism Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + O2 + reduced putidaredoxin
-
Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
3-chloroperbenzoic acid + O2 + reduced putidaredoxin reaction mechanism of substrate-free ferric cytochrome P450cam, via FeIV-O plus porphyrin Pi-cation radical, overview Pseudomonas putida ?
-
?
peracetic acid + O2 + reduced putidaredoxin reaction mechanism of substrate-free ferric cytochrome P450cam, via FeIV-O plus tyrosyl radical, overview Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
cytochrome p450cam
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4
-
assay at 3.4°C and 25°C Pseudomonas putida
25
-
assay at 3.4°C and 25°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 8 assay at pH 6.2, pH 7.0, pH 7.4, and pH 8.0 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
putidaredoxin
-
Pseudomonas putida