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Literature summary for 1.14.15.1 extracted from
- Conformational heterogeneity and the affinity of substrate molecular recognition by cytochrome P450cam (2017), Biochemistry, 56, 3248-3256 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C334A | site-directed mutagenesis, the mutation reduces protein aggregation, but has no effect on catalytic activity | Pseudomonas putida |
| Y96C/C334A | site-directed mutagenesis, substrate recognition and binding compared to the wild-type, conformational selection mechanism | Pseudomonas putida |
| Y96F/C334A | site-directed mutagenesis, substrate recognition and binding compared to the wild-type, conformational selection mechanism | Pseudomonas putida |
| Y96N/C334A | site-directed mutagenesis, substrate recognition and binding compared to the wild-type, conformational selection mechanism | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | thermodynamics of wild-type and mutant enzymes with different substrates, overview | Pseudomonas putida |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | in heme | Pseudomonas putida |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | P00183 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by a process including anion exchange chromatography and gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
| camphane + reduced putidaredoxin + O2 | - |
Pseudomonas putida | ? + oxidized putidaredoxin + H2O | - |
? | |
| isoborneol + reduced putidaredoxin + O2 | - |
Pseudomonas putida | ? + oxidized putidaredoxin + H2O | - |
? | |
| additional information | analysis of the molecular substrate recognition of wild-type and mutant P450cams by infrared (IR) spectroscopy, differing conformational heterogeneity in the active site of the P450cam variants and changes in heterogeneity upon binding of different substrates likely contribute to their variable affinities via a conformational selection mechanism, overview. Although P450cam is relatively specific for camphor, it also recognizes a number of small, camphor-like substrates, albeit with variable affinity. Substrate binding and active site structure, overview | Pseudomonas putida | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome p450cam | - |
Pseudomonas putida |
| P450cam | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Pseudomonas putida | |
| heme | - |
Pseudomonas putida | |
| putidaredoxin | - |
Pseudomonas putida |
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