Cloned (Comment) | Organism |
---|---|
expressed in Rhodococcus jostii strain RHA1 | Mycobacterium tuberculosis |
expressed in Rhodococcus jostii strain RHA1 | Mycobacterium tuberculosis variant bovis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
O2 | apparent value, Km above 1.2 mM, in 0.1 M potassium phosphate at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46400 | - |
MALDI-TOF mass spectrometry | Mycobacterium tuberculosis |
48200 | - |
MALDI-TOF mass spectrometry | Mycobacterium tuberculosis variant bovis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Mycobacterium tuberculosis variant bovis | - |
- |
- |
Mycobacterium tuberculosis variant bovis bacillus Calmette-Guerin | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Source 15Q column chromatography | Mycobacterium tuberculosis |
Source 15Q column chromatography | Mycobacterium tuberculosis variant bovis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis | 26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis variant bovis | 26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis variant bovis bacillus Calmette-Guerin | 26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis H37Rv | 26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis | 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis variant bovis | 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis variant bovis bacillus Calmette-Guerin | 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
cholesterol + reduced ferredoxin [iron-sulfur] cluster + O2 | hydroxylation occurs at carbon 26 of the steroid side chain | Mycobacterium tuberculosis H37Rv | 26-hydroxycholesterol + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP125 | - |
Mycobacterium tuberculosis |
CYP125 | - |
Mycobacterium tuberculosis variant bovis |
cytochrome P450 125 | - |
Mycobacterium tuberculosis |
cytochrome P450 125 | - |
Mycobacterium tuberculosis variant bovis |
Rv3545c | - |
Mycobacterium tuberculosis |
Rv3545c | - |
Mycobacterium tuberculosis variant bovis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme is heat inactivated by incubation at 65°C for 30 min | Mycobacterium tuberculosis |
65 | - |
the enzyme is heat inactivated by incubation at 65°C for 30 min | Mycobacterium tuberculosis variant bovis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis | |
NADH | - |
Mycobacterium tuberculosis variant bovis |
Organism | Comment | Expression |
---|---|---|
Mycobacterium tuberculosis variant bovis | cyp125 is up-regulated 7.1fold with growth on cholesterol | up |
General Information | Comment | Organism |
---|---|---|
physiological function | Cyp125 is essential for the growth of Mycobacterium bovis strain bacillus Calmette-Guerin on cholesterol | Mycobacterium tuberculosis variant bovis |
physiological function | Cyp125 is not essential for the growth of Mycobacterium tuberculosis on cholesterol | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11000 | - |
O2 | apparent value, in 0.1 M potassium phosphate at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis |