General Stability | Organism |
---|---|
after 24 h 15 mM EDTA 60% activity and iron retained | Pseudomonas oleovorans |
after 4 h 15 mM EDTA and 10 mM dithionite 10% activity and iron retained | Pseudomonas oleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | reversible inhibitor | Pseudomonas oleovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain | Pseudomonas oleovorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | 0.03 atoms per 42000 g/mol peptide | Pseudomonas oleovorans | |
Fe | 1 atom Fe per 42000 g/mol peptide | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40100 | - |
x * 40100, amino acid composition | Pseudomonas oleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
n-alkane + NAD(P)H + H+ + O2 | Pseudomonas oleovorans | - |
n-alkanol + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 5% carbohydrate | Pseudomonas oleovorans |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas oleovorans |
Renatured (Comment) | Organism |
---|---|
ferrous ions restore activity after treatment with EDTA and dithionite | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.23 | - |
hydroxylation of octane | Pseudomonas oleovorans |
1.75 | - |
epoxidation of 1,7-octadiene | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,7-octadiene + NADH + H+ + O2 | epoxidation of simple, aliphatic terminal olefins | Pseudomonas oleovorans | 1,2-epoxy-7-octene + NAD+ + H2O | - |
? | |
fatty acid + NAD(P)H + H+ + O2 | - |
Pseudomonas oleovorans | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | - |
Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | rubredoxin is electron carrier | Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40100, amino acid composition | Pseudomonas oleovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | hydroxylation needs also a NADH-dependent bacterial reductase | Pseudomonas oleovorans |