Cloned (Comment) | Organism |
---|---|
the 1.4 kb putative promoter region upstream of the predicted CYP86A1 start codon fused in frame to the beta-glucuronidase gene and used to transform wild-type Arabidopsis under the control of the native promoter. Transgenic expression of CYP86A1 fused to GFP, CYP86A1:GFP heterologously and transiently expressed under the control of the CaMV35S-promoter in the epidermis of Nicotiana benthamiana leaves | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | transgenic expression of CYP86A1 fused to GFP distributes CYP86A1 to the endoplasmic reticulum, indicating that suberin monomer biosynthesis takes place in this sub-cellular compartment before intermediates are exported in the apoplast | Arabidopsis thaliana | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P48422 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no detectable CYP86A1 transcript levels in above-ground organs such as leaves, stems, flowers, and siliques | Arabidopsis thaliana | - |
root | strong root specificity and a localized expression in the root endodermis, high expression levels in all three root zones from the tip to the base | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
suberin + NADPH + H+ + O2 | - |
Arabidopsis thaliana | ? + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP86A1 | - |
Arabidopsis thaliana |
Fatty acid omega-hydroxylase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | is a key enzyme for aliphatic root suberin biosynthesis. In contrast to wild-type, no CYP86A1 transcript in total RNA from horst-1 seedlings (carries the T-DNA insertion in the first exon of CYP86A1) and horst-2 seedlings (T-DNA insertion is located in the second exon). Complemented CYP86A1-transformed horst-1 plants reveal a quantitative and qualitative aliphatic suberin composition similar to the wild-type | Arabidopsis thaliana |