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Literature summary for 1.14.15.3 extracted from

  • Liu, H.; Xu, J.; Liang, R.; Liu, J.
    Characterization of the medium- and long-chain n-alkanes degrading Pseudomonas aeruginosa strain SJTD-1 and its alkane hydroxylase genes (2014), PLoS ONE, 9, e105506 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
degradation strain SJTD-1 efficiently mineralizes medium- and long-chain n-alkanes (C12-C30) as its sole carbon source within seven days, showing optimal growth on n-hexadecane, followed by n-octadecane, and n-eicosane. In 36 h, 500 mg/l of tetradecane, hexadecane, and octadecane are transformed completely; and 2 g/l n-hexadecane is degraded to undetectable levels within 72 h Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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Pseudomonas aeruginosa SJTD-1
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Subunits

Subunits Comment Organism
? x * 42100, calculated for isoform AlkB1, x * 41600, calculated for isoform AlkB2 Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa expression of AlkB1 is induced by C12-C16 alkanes, expression of AlkB2 by C12-C24 alkanes up

General Information

General Information Comment Organism
physiological function for the medium-chain n-alkanes (C12-C16), single-knockout mutants of isoforms AlkB1 and AlkB2 show an obvious delay as compared to the wild type SJTD-1, and double-knockout mutants cannot utilize the n-alkanes at all. The loss of AlkB2 show a more pronounced effect on the cell growth than the loss of AlkB1. The poor viability of the double mutant recovers to a normal state when C18-C24 alkanes are used as the sole source of carbon Pseudomonas aeruginosa