Cloned (Comment) | Organism |
---|---|
gene A5N73_13410, sequence comparisons | Rhodococcus equi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 by a PCR-targeted gene disruption method results in a mutant strain that accumulates up to 0.58 mg/ml 1,4-androstadiene-3,17-dione in the culture medium when 0.2% cholesterol is used as the carbon source. The mutant also accumulates 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (DELTA1,4-BNC). 1,4-Androstadiene-3,17-dione in strain USA-18DELTAB8 preferentially imposes a feedback inhibition on the enzymes involved in the side chain oxidation of DELTA1,4-BNC, but not DELTA4-BNC. Mutant phenotypes, overview | Rhodococcus equi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Rhodococcus equi | - |
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Rhodococcus equi 103S | - |
9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Rhodococcus equi | - |
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | Rhodococcus equi 103S | - |
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus equi | A0A060CNE1 | i.e. Rhodococcus hoagii | - |
Rhodococcus equi 103S | A0A060CNE1 | i.e. Rhodococcus hoagii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus equi | 9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
3-oxo-23,24-bisnorchola-1,4-dien-22-oate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus equi 103S | 9alpha-hydroxy-3-oxo-23,24-bisnorchol-4-en-22-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus equi | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Rhodococcus equi 103S | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid 9alpha-hydroxylase | - |
Rhodococcus equi |
A5N73_13410 | - |
Rhodococcus equi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Rhodococcus equi |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the gene encoding the reductase component KshB of 3-ketosteroid 9alpha-hydroxylase in Rhodococcus equi strain USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione | Rhodococcus equi |