Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.32 extracted from

  • Quaderer, R.; Omura, S.; Ikeda, H.; Cane, D.
    Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis (2006), J. Am. Chem. Soc., 128, 13036-13037.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells as a C-terminal-His6-tag protein Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00333
-
 (+/-)-pentalenene recombinant enzyme, in 50 mM phosphate buffer, 10% (v/v) glycerol, pH 7.4, at 22°C Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-pentalenene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Streptomyces avermitilis pentalen-13-al + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 r

Synonyms

Synonyms Comment Organism
CYP183A1
-
 Streptomyces avermitilis
PtlI
-
 Streptomyces avermitilis
SAV2999
-
 Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00838
-
 (+/-)-pentalenene recombinant enzyme, in 50 mM phosphate buffer, 10% (v/v) glycerol, pH 7.4, at 22°C Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Streptomyces avermitilis