Literature summary for 1.14.15.33 extracted from

Han, S.; Pham, T.V.; Kim, J.H.; Lim, Y.R.; Park, H.G.; Jeong, D.; Yun, C.H.; Chun, Y.J.; Kang, L.W.; Kim, D.
Structural insights into the binding of lauric acid to CYP107L2 from Streptomyces avermitilis (2017), Biochem. Biophys. Res. Commun., 482, 902-908 .

Search for more literature for 1.14.15.33

Cloned(Commentary)

Cloned (Comment)	Organism
gene cyp107l2, recombinant expression of His-tagged enzyme in Escherichia coli	Streptomyces avermitilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant ligand-free CYP107L2 and its complex with lauric acid, (1) sitting drop vapor diffusion method, mixing of 500 nl of 12 mg/ml protein solution with 500 nl of reservoir solution containing 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% w/v PEG 8000, and 15% v/v glycerol, for complex crystals lauric acid in a 1:10 M ratio, and equilibration against 0.05 ml of reservoir solution, 14°C, 30 days, (2) hanging drop vapor diffusion method, mixing of 0.001 ml of 12 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% w/v PEG 8000, and 15% v/v glycerol, and equilibration against 0.05 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.5-2.6 A resolution	Streptomyces avermitilis

Crystallization (Comment)

Organism

purified recombinant ligand-free CYP107L2 and its complex with lauric acid, (1) sitting drop vapor diffusion method, mixing of 500 nl of 12 mg/ml protein solution with 500 nl of reservoir solution containing 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% w/v PEG 8000, and 15% v/v glycerol, for complex crystals lauric acid in a 1:10 M ratio, and equilibration against 0.05 ml of reservoir solution, 14°C, 30 days, (2) hanging drop vapor diffusion method, mixing of 0.001 ml of 12 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% w/v PEG 8000, and 15% v/v glycerol, and equilibration against 0.05 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.5-2.6 A resolution

Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	Streptomyces avermitilis	-	novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	Streptomyces avermitilis ATCC 31267	-	novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?
narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	Streptomyces avermitilis	-	pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	Streptomyces avermitilis ATCC 31267	-	pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces avermitilis	Q82LM3	-	-
Streptomyces avermitilis ATCC 31267	Q82LM3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	no catalytic activity with lauric acid. The lauric acid is bound mainly via hydrophobic interactions with the carboxylate group of lauric acid coordinated to the heme of P450. Residue Glu40 and Leu382 in the CYP107L2 complex with lauric acid show significant conformational changes to provide plentiful room for the lauric acid in the substrate-binding site. Binding structure of enzyme, lauric acid and pikromycin, overview	Streptomyces avermitilis	?	-	?
additional information	no catalytic activity with lauric acid. The lauric acid is bound mainly via hydrophobic interactions with the carboxylate group of lauric acid coordinated to the heme of P450. Residue Glu40 and Leu382 in the CYP107L2 complex with lauric acid show significant conformational changes to provide plentiful room for the lauric acid in the substrate-binding site. Binding structure of enzyme, lauric acid and pikromycin, overview	Streptomyces avermitilis ATCC 31267	?	-	?
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	-	Streptomyces avermitilis	novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	-	Streptomyces avermitilis ATCC 31267	novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?
narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Streptomyces avermitilis	pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Streptomyces avermitilis ATCC 31267	pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 43000, recombinant His-tagged enzyme, SDS-PAGE and crystal structure analysis	Streptomyces avermitilis

Synonyms

Synonyms	Comment	Organism
CYP107L2	-	Streptomyces avermitilis
PikC	-	Streptomyces avermitilis

Cofactor

Cofactor	Comment	Organism
cytochrome P450	-	Streptomyces avermitilis
ferredoxin [iron-sulfur] cluster	-	Streptomyces avermitilis
heme	CYP107L2 shows a low-spin state of heme. Heme is sandwiched between helices I and L in the conserved way of P450 structures. The I-helix crosses the center of CYP107L2 in a slightly bent form over the heme structure, while helices F and G are stacked onto the I-helix to form a wide-open substrate-binding cavity just above the heme moiety	Streptomyces avermitilis

General Information

General Information	Comment	Organism
physiological function	CYP107L1 catalyzes the hydroxylation of narbomycin to produce pikromycin and novapikromycin	Streptomyces avermitilis