Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCN | slight | Curvularia lunata | |
MnCl2 | causes most of activity to disappear in crude extracts | Curvularia lunata | |
NAD(P)H | enhance activity of crude extracts that show hydroxylation without any cofactor | Curvularia lunata |
General Stability | Organism |
---|---|
alpha-lipoic acid, during DEAE chromatography stabilizes | Curvularia lunata |
EDTA, stabilizes during purification | Curvularia lunata |
GSH, stabilizes during purification, as well as other sulfhydryl compounds | Curvularia lunata |
Tween 80, positive influence on activity conservation during extraction and fractionation | Curvularia lunata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Metyrapone | 2 mM | Curvularia lunata | |
p-chloromercuribenzoate | 1 mM | Curvularia lunata | |
phenazine methosulfate | 1 mM, crude extract | Curvularia lunata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | - |
Boedijn NRRL 2380 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, ion-exchange chromatography | Curvularia lunata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | both hydroxylation activities seem to occur in a single enzyme | Curvularia lunata |
Storage Stability | Organism |
---|---|
-30°C, extract precipitate stable, solution less stable, DEAE fraction unstable | Curvularia lunata |
-30°C, preserved by Tween 80, conservation of activity | Curvularia lunata |
0°C, preserved by Tween 80, conservation of activity | Curvularia lunata |
2°C, crude extract loses 50% activity in 24 h | Curvularia lunata |
purified or partially purified enzyme unstable during storage | Curvularia lunata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha,21-dihydroxy-pregn-4-ene-3,20-dione + reduced adrenal ferredoxin + O2 | i.e. Reichstein's compound, inducer | Curvularia lunata | 11beta,17alpha,21-trihydroxy-pregn-4-ene-3,20-dione + oxidized adrenal ferredoxin + H2O | hydrocortisone, 60%, 11beta hydroxylation | ? | |
17alpha,21-dihydroxy-pregn-4-ene-3,20-dione + reduced adrenal ferredoxin + O2 | i.e. Reichstein's compound, inducer | Curvularia lunata | 14alpha,17alpha,21-trihydroxy-pregn-4-ene-3,20-dione + oxidized adrenal ferredoxin + H2O | 25%, 14alpha hydroxylation | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Curvularia lunata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | 15 | 2fold decrease of activity with increase of temperature, temperatures above 25°C unfavourable | Curvularia lunata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
crude extract, 7 mg/ml, 24 h, loss of half the activity, DEAE fraction unstable | Curvularia lunata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Curvularia lunata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Curvularia lunata |