Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | affects 11beta-/19-hydroxylase reaction | Bos taurus | |
Metyrapone | competitive with substrate, affects strongly 11beta-/18-hydroxylation and 11beta-/19-hydroxylation | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
deoxycorticosterone | identical for both enzyme activities | Bos taurus | |
0.013 | - |
4-androstene-3,17-dione | identical for both enzyme activities | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | P-45011beta responsible for two hydroxylase reactions | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of sonicated mitochondria and cholate extract of hydroxylation activities with deoxycotricosterone and 4-androstene-3,17-dione as substrates | Bos taurus |
Storage Stability | Organism |
---|---|
-80°C, 59 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM deoxycorticosterone, 0.3% cholate, 0.3% Tween 20 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2 | i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate | Bos taurus | 11beta-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O | - |
? | |
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2 | i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate | Bos taurus | 19-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
11-deoxycorticosterone | 18-hydroxylation | Bos taurus | |
1.83 | - |
11-deoxycorticosterone | 11beta-hydroxylation | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |