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Literature summary for 1.14.15.4 extracted from

  • Delorme, C.; Piffeteau, A.; Sobrio, F.; Marquet, A.
    Mechanism-based inactivation of bovine cytochrome P-45011beta by 18-unsaturated progesterone derivatives (1997), Eur. J. Biochem., 248, 252-260.
    View publication on PubMed
Search for more literature for 1.14.15.4

Application

Application Comment Organism
medicine inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension Bos taurus

General Stability

General Stability Organism
deoxycorticosterone, protection of enzyme from inactivation by 18-ethynylprogesterone Bos taurus
phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
18-ethynylprogesterone mechanism-based inhibition; progesterone analog, time-dependent pseudo-first-order inactivation, concentration dependent Bos taurus
18-vinylprogesterone mechanism-based inhibition; progesterone analog, with NADPH, time and concentration dependent, irreversible, pseudo-first-order process, covalent binding to and destruction of prosthetic heme group, 5fold more effective than its acetylenic analog 18-ethynylprogesterone Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
-80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 3 step 11beta-/18-hydroxylation to aldosterone Bos taurus aldosterone + oxidized adrenal ferredoxin + 2 H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH requirement during inactivation Bos taurus
NADPH adrenodoxin and NADPH-linked adrenodoxin reductase Bos taurus