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Literature summary for 1.14.15.4 extracted from

  • Ohnishi, T.; Miura, S.; Ichikawa, Y.
    Photoaffinity labeling of cytochrome P-45011beta with methyltrienolone as a probe for the substrate binding region (1993), Biochim. Biophys. Acta, 1161, 257-264.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
methyltrienolone synthetic androgen, used as photoaffinity ligand and substrate analog, covalent binding, 0.1 mM inhibits cortisol synthesis, during photolabeling radioactivity incorporation via radioactive methyltrienolone is blocked by 11-deoxycorticosterone, so it binds to the conserved substrate binding region Trp428-Leu429-Asp430-Arg431 between beta3-sheet and the L-helix analysed by trypsin digest Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
11-deoxycorticosterone presence of methyltrienolone Bos taurus
0.002
-
11-deoxycorticosterone absence of methyltrienolone Bos taurus
0.06 0.09 corticosterone presence of methyltrienolone Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
RP HPLC for detection of photolabeled peptide after digest with TPCK-treated trypsin of purified cytochrome P-45011beta, which is photolabeled with radioactive methyltrienolone Bos taurus

Reaction

Reaction Comment Organism Reaction ID
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O superfamily of heme-containing enzyme, 11-/18-/19-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstenedione Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
Bos taurus
-

Storage Stability

Storage Stability Organism
-80°C, 20 mM potassium phosphate buffer, pH 7.4, 0.01 mM 11-deoxycorticosterone, 0.1 mM EDTA, 0.1 mM dithiothreitol, 1%, w/v, sodium cholate Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycortisol + reduced adrenal ferredoxin + O2 cytochrome P-45011beta-linked monoxygenase Bos taurus cortisol + oxidized adrenal ferredoxin + H2O
-
?

Synonyms

Synonyms Comment Organism
cytochrome P-45011-beta
-
Bos taurus
P-450XIB1
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH adrenodoxin and NADPH-linked adrenodoxin reductase Bos taurus