Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methyltrienolone | synthetic androgen, used as photoaffinity ligand and substrate analog, covalent binding, 0.1 mM inhibits cortisol synthesis, during photolabeling radioactivity incorporation via radioactive methyltrienolone is blocked by 11-deoxycorticosterone, so it binds to the conserved substrate binding region Trp428-Leu429-Asp430-Arg431 between beta3-sheet and the L-helix analysed by trypsin digest | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
11-deoxycorticosterone | presence of methyltrienolone | Bos taurus | |
0.002 | - |
11-deoxycorticosterone | absence of methyltrienolone | Bos taurus | |
0.06 | 0.09 | corticosterone | presence of methyltrienolone | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RP HPLC for detection of photolabeled peptide after digest with TPCK-treated trypsin of purified cytochrome P-45011beta, which is photolabeled with radioactive methyltrienolone | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | superfamily of heme-containing enzyme, 11-/18-/19-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstenedione | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-80°C, 20 mM potassium phosphate buffer, pH 7.4, 0.01 mM 11-deoxycorticosterone, 0.1 mM EDTA, 0.1 mM dithiothreitol, 1%, w/v, sodium cholate | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycortisol + reduced adrenal ferredoxin + O2 | cytochrome P-45011beta-linked monoxygenase | Bos taurus | cortisol + oxidized adrenal ferredoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P-45011-beta | - |
Bos taurus |
P-450XIB1 | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus |