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Literature summary for 1.14.15.6 extracted from

  • Hanukoglu, I.; Jefcoate, C.R.
    Mitochondrial cytochrome P-450sec. Mechanism of electron transport by adrenodoxin (1980), J. Biol. Chem., 255, 3057-3061.
    View publication on PubMed
Search for more literature for 1.14.15.6

Inhibitors

Inhibitors Comment Organism Structure
adrenodoxin oxidized form, high affinity to P-450scc, inhibits side chain cleavage by competition with reduced form Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 reduced adrenodoxin free Bos taurus
0.00056
-
 reduced adrenodoxin +/-0.00004, complex between adrenodoxin and adrenodoxin reductase Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane integral to membrane Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
 ?
cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
cholesterol + reduced adrenodoxin + O2 + H+
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 r
additional information suggested shuttle mechanism in which adrenodoxin transfers electrons between adrenodoxin reductase and cytochrome P-450. No ternary adrenodoxin-adrenodoxin reductase-P-450 complex Bos taurus ?
-
 ?
additional information reduction of adrenodoxin must be rate-limiting step Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
adrenodoxin transports electrons from adrenodoxin reductase to cytochrome P-450 by shuttling Bos taurus
NADPH NADPH-linked adrenodoxin reductase Bos taurus