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Literature summary for 1.14.15.6 extracted from
- The catalytic cycle of cytochrome P-450scc and intermediates in the conversion of cholesterol to pregnenolone (1984), Eur. J. Biochem., 140, 583-591.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increased rate of association of cholesterol to partially purified cytochrome P-450scc | Bos taurus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| iso-octane and ammonium sulfate fractionating, gel filtration | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal cortex | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
| cholesterol + reduced adrenodoxin + O2 | stable intermediates: 22-hydroxycholesterol and 20,22-dihydroxycholesterol | Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adrenodoxin | - |
Bos taurus | |
| heme | haemoprotein | Bos taurus | |
| NADPH | NADPH-linked adrenodoxin reductase | Bos taurus | |
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Release 2023.1 (January 2023)
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