Literature summary for 1.14.15.6 extracted from

Minenko, A.N.; Novikova, L.A.; Luzikov, V.N.; Kovaleva, I.E.
Import of hybrid forms of CYP11A1 into yeast mitochondria (2008), Biochim. Biophys. Acta, 1780, 1121-1130.

Search for more literature for 1.14.15.6

Cloned(Commentary)

Cloned (Comment)	Organism
hybrid proteins AAC-mCYP11A1, Bcs1p(183)-mCYP11A1, Su9(1-116)-mCYP11A1, preAd-mCYP11A1, DLD(1-72)-mCYP11A1 and COXIV-mCYP11A1 expressed in Saccharomyces cerevisiae strain 2805. Escherichia coli strain JM109 transformed with the plasmid pTrc99(A)/Ad-mCYP11A1	synthetic construct

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	all of the addressing sequences, containing transmembrane domains, provide effective insertion of the hybrid proteins AAC-mCYP11A1, Bcs1p(1-83)-mCYP11A1, DLD(1-72)-mCYP11A1 and Su9(1-116)-mCYP11A1 into the mitochondrial inner membrane. preAd-mCYP11A1 hybrid molecules are translocated across the inner membrane and tightly associated with the membrane on its matrix side but not membrane inserted. The mechanism of Ad-mCYP11A1 hybrid topogenesis in Escherichia coli cells differs from that of the topogenesis of its precursor form in yeast mitochondria	synthetic construct	-	-

Organism

Organism	UniProt	Comment	Textmining
synthetic construct	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
22(R)-hydroxycholesterol + reduced adrenodoxin + O2	mitochondria containing the AAC-mCYP11A1 hybrid have the highest cholesterol side-chain cleavage activity in the reconstituted system. Ad-mCYP11A1 hybrid demonstrates only residual enzyme activity	synthetic construct	? + oxidized adrenodoxin + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP11A1	-	synthetic construct
cytochrome P450scc	-	synthetic construct

Cofactor

Cofactor	Comment	Organism	Structure
adrenodoxin	-	synthetic construct

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Release 2023.1 (January 2023)