Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | vitamin D3 and cholesterol are metabolized with comparable efficiencies in cyclodextrin, but the Km for both show a strong dependence on the cyclodextrin concentration, decreasing with decreasing cyclodextrin | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
cholesterol | in the presence of 0.45% cyclodextrin | Bos taurus | |
0.0296 | - |
vitamin D3 | in the presence of 0.45% cyclodextrin | Bos taurus | |
0.067 | - |
20-hydroxyvitamin D3 | in the presence of 0.45% cyclodextrin | Bos taurus | |
1.18 | - |
cholesterol | in the presence of 4.5% cyclodextrin | Bos taurus | |
3.67 | - |
vitamin D3 | in the presence of 4.5% cyclodextrin | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bos taurus | 16020 | - |
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20,23-dihydroxyvitamin D3 + reduced adrenodoxin + O2 | - |
Bos taurus | 17,20,23-trihydroxyvitamin D3 + oxidized adrenodoxin + H2O | - |
? | |
20-hydroxyvitamin D3 + reduced adrenodoxin + O2 | - |
Bos taurus | 20,23-dihydroxyvitamin D3 + oxidized adrenodoxin + H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
vitamin D3 + reduced adrenodoxin + O2 | is hydroxylated with lower efficiency than cholesterol | Bos taurus | 20-hydroxyvitamin D3 + oxidized adrenodoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP11A1 | - |
Bos taurus |
cytochrome P450scc | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
cholesterol | in the presence of 0.45% cyclodextrin | Bos taurus | |
0.123 | - |
20-hydroxyvitamin D3 | in the presence of 0.45% cyclodextrin | Bos taurus | |
0.248 | - |
cholesterol | in the presence of 4.5% cyclodextrin | Bos taurus | |
0.328 | - |
vitamin D3 | in the presence of 0.45% cyclodextrin | Bos taurus | |
1.6 | - |
vitamin D3 | in the presence of 4.5% cyclodextrin | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.212 | - |
cholesterol | in the presence of 4.5% cyclodextrin | Bos taurus | |
0.435 | - |
vitamin D3 | in the presence of 4.5% cyclodextrin | Bos taurus | |
1.83 | - |
20-hydroxyvitamin D3 | in the presence of 0.45% cyclodextrin | Bos taurus | |
11.1 | - |
vitamin D3 | in the presence of 0.45% cyclodextrin | Bos taurus | |
11.17 | - |
cholesterol | in the presence of 0.45% cyclodextrin | Bos taurus |