Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rubrivivax gelatinosus |
expression in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
spheroidene | pH 7.8, 28°C | Rubrivivax gelatinosus | |
0.009 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2,2'-dioxospririlloxanthin | Rubrivivax gelatinosus | |
0.0101 | - |
1'-hydroxyspheroidene | pH 7.8, 28°C | Rhodobacter capsulatus | |
0.0136 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2,2'-diketospririlloxanthin | Rhodobacter capsulatus | |
0.0155 | - |
1'-hydroxyspheroidene | pH 7.8, 28°C | Rubrivivax gelatinosus | |
0.0169 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2-ketospririlloxanthin | Rhodobacter capsulatus | |
0.0181 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2-oxospririlloxanthin | Rubrivivax gelatinosus | |
0.0191 | - |
spheroidene | pH 7.8, 28°C | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Rubrivivax gelatinosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rubrivivax gelatinosus |
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2 | conversion rate: 28% | Rhodobacter capsulatus | 1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2 | conversion rate: 37% | Rubrivivax gelatinosus | 1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
1'-hydroxyspheroidene + reduced ferredoxin + O2 | conversion rate: 41% | Rhodobacter capsulatus | 1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
1'-hydroxyspheroidene + reduced ferredoxin + O2 | conversion rate: 76% | Rubrivivax gelatinosus | 1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
1,1'-dihydroxy-3,4-didehydrolycopene + 2 reduced ferredoxin + 2 O2 | - |
Rubrivivax gelatinosus | 2,2'-dioxo-1,1'-dihydroxy-3,4-didehydrolycopene + 2 oxidized ferredoxin + 2 H2O | - |
? | |
1-hydroxy-3,4-didehydrolycopene + reduced ferredoxin + O2 | - |
Rubrivivax gelatinosus | 2-oxo-1-hydroxy-3,4-didehydrolycopene + oxidized ferredoxin + H2O | - |
? | |
2-hydroxyspheroidene + reduced ferredoxin + O2 | - |
Rubrivivax gelatinosus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
2-hydroxyspheroidene + reduced ferredoxin + O2 | - |
Rhodobacter capsulatus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
spheroidene + reduced ferredoxin + O2 | conversion rate: 36% | Rhodobacter capsulatus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
spheroidene + reduced ferredoxin + O2 | conversion rate: 68% | Rubrivivax gelatinosus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
spirilloxanthin + 2 reduced ferredoxin + 2 O2 | conversion rate: 41% | Rhodobacter capsulatus | 2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O | - |
? | |
spirilloxanthin + 2 reduced ferredoxin + 2 O2 | conversion rate: 43% | Rubrivivax gelatinosus | 2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O | - |
? | |
spirilloxanthin + reduced ferredoxin + O2 | conversion rate: 59% | Rubrivivax gelatinosus | 2-oxospirilloxanthin + oxidized ferredoxin + H2O | - |
? | |
spirilloxanthin + reduced ferredoxin + O2 | conversion rate: 59% | Rhodobacter capsulatus | 2-oxospirilloxanthin + oxidized ferredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
acyclic carotenoid 2-ketolase | - |
Rubrivivax gelatinosus |
acyclic carotenoid 2-ketolase | - |
Rhodobacter capsulatus |
CrtA | - |
Rubrivivax gelatinosus |
CrtA | - |
Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Rubrivivax gelatinosus |
28 | - |
assay at | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rubrivivax gelatinosus |
7.8 | - |
assay at | Rhodobacter capsulatus |