Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | in the absence of dithiothreitol the activity of recently purified PheH is 50% of that achieved when dithiothreitol is present | Chromobacterium violaceum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chromobacterium violaceum |
Protein Variants | Comment | Organism |
---|---|---|
I234D | mutant shows decreased kcat value for 6,7-dimethyltetrahydropterin compared to the wild type enzyme | Chromobacterium violaceum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | in the absence of added iron in the assay, the EDTA-treated PheH exhibits 3.5% of the L-phenylalanine hydroxylation activity of the metal-containing enzyme | Chromobacterium violaceum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | PheH is a non-heme iron monooxygenase | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-Sephacel gel filtration | Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 | - |
Chromobacterium violaceum | ? | - |
? | |
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 | - |
Chromobacterium violaceum | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-phenylalanine + 6,7-dimethyltetrahydropterin + O2 | - |
Chromobacterium violaceum | 4-(hydroxymethyl)phenylalanine + 3-methyltyrosine + H2O + 6,7-dimethyl-dihydropterin | - |
? | |
L-phenylalanine + 6-methyltetrahydropterin + O2 | - |
Chromobacterium violaceum | L-tyrosine + 2-amino-4a-hydroxy-7-methyl-5,6,7,8-tetrahydropteridin-4(4aH)-one | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheH | - |
Chromobacterium violaceum |
phenylalanine hydroxylase | - |
Chromobacterium violaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
6,7-dimethyltetrahydropterin | mutant enzyme I234D, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol | Chromobacterium violaceum | |
1.4 | - |
6,7-dimethyltetrahydropterin | wild type enzyme, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol | Chromobacterium violaceum | |
1.6 | - |
6-methyltetrahydropterin | wild type enzyme, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol | Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Chromobacterium violaceum |