Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected in any direction by preincubation with the substrate L-phenylalanine | Legionella pneumophila |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
tetrahydrobiopterin | in 100 mM Na-HEPES, pH 7.0 at 37°C | Legionella pneumophila | |
0.735 | - |
L-phenylalanine | in 100 mM Na-HEPES, pH 7.0 at 37°C | Legionella pneumophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, SDS-PAGE | Legionella pneumophila |
32500 | - |
2 * 32500, calculated from amino acid sequence | Legionella pneumophila |
64000 | - |
gel filtration | Legionella pneumophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | Legionella pneumophila | - |
L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | Legionella pneumophila 130b | - |
L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | - |
- |
- |
Legionella pneumophila 130b | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON resin column chromatography and Superdex 200 gel filtration | Legionella pneumophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.2 | - |
in 100 mM Na-HEPES, pH 7.0 at 37°C | Legionella pneumophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Legionella pneumophila | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Legionella pneumophila 130b | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31000, SDS-PAGE | Legionella pneumophila |
homodimer | 2 * 32500, calculated from amino acid sequence | Legionella pneumophila |
Synonyms | Comment | Organism |
---|---|---|
PAH | - |
Legionella pneumophila |
phenylalanine hydroxylase | - |
Legionella pneumophila |
phhA | - |
Legionella pneumophila |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme has a major functional role in the synthesis of pyomelanin and promotes growth in media lacking tyrosine | Legionella pneumophila |