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Literature summary for 1.14.16.1 extracted from

  • Roberts, K.M.; Khan, C.A.; Hinck, C.S.; Fitzpatrick, P.F.
    Activation of phenylalanine hydroxylase by phenylalanine does not require binding in the active site (2014), Biochemistry, 53, 7846-7853 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
L-phenylalanine
-
Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R270K the mutation effectively abolishes binding of L-phenylalanine in the active site and leads to strongly reduced kcat/Km value (10000 down) compared to the wild type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
500
-
L-phenylalanine mutant enzyme R270K, at pH 7.0 and 25°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 Rattus norvegicus
-
L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography and Q Sepharose column chromatography, and Superdex 200 gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
Rattus norvegicus L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
?

Synonyms

Synonyms Comment Organism
PheH
-
Rattus norvegicus
phenylalanine hydroxylase
-
Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
28.3
-
L-phenylalanine mutant enzyme R270K, at pH 7.0 and 25°C Rattus norvegicus