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Literature summary for 1.14.16.4 extracted from

  • Ogawa, S.; Ichinose, H.
    Effect of metals and phenylalanine on the activity of human tryptophan hydroxylase-2: comparison with that on tyrosine hydroxylase activity (2006), Neurosci. Lett., 401, 261-265.
    View publication on PubMed
Search for more literature for 1.14.16.4

Cloned(Commentary)

Cloned (Comment) Organism
expression as maltose-binding protein fusion protein in Escherichia coli BL21 (DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 80% inhibition at 0.1 mM Homo sapiens
L-phenylalanine
-
 Homo sapiens
Mn2+ 50% inhibition at 0.01 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0132
-
 L-tryptophan 0.02 mM ferrous ammonium, 0.5 mM (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin, 30°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 SDS-PAGE, maltose-binding protein fusion protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IWU9
-
-

Purification (Commentary)

Purification (Comment) Organism
purified by amylose resin affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2
-
 Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Synonyms

Synonyms Comment Organism
hTPH2
-
 Homo sapiens
tryptophan hydroxylase-2
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0727
-
 L-phenylalanine 0.02 mM ferrous ammonium, 0.5 mM (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin, 30°C Homo sapiens