Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adrenocorticotrophic hormone(1-10) | - |
Rattus norvegicus | |
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) | - |
Rattus norvegicus | |
endorphin(51-61)NH2 | i.e. pro-adrenocorticotrophic hormone(1-10) | Rattus norvegicus | |
endorphins | and proendorphins | Rattus norvegicus | |
peptides | especially those with COOH-terminal glycine residues | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
secretory granule | - |
Rattus norvegicus | 30141 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | Cu2+ strongly stimulates activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pituitary gland | anterior | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
very low activity in crude subcellular preparations | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2 | inhibitory substrate | Rattus norvegicus | alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O | - |
r | |
D-Tyr-Val-Gly + ascorbate + O2 | synthetic peptid | Rattus norvegicus | D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
monoiodo-D-Tyr-Val-Gly + ascorbate + O2 | synthetic peptid | Rattus norvegicus | monoiodo-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
additional information | specificity | Rattus norvegicus | ? | - |
? | |
additional information | endorphins are inhibitory substrates | Rattus norvegicus | ? | - |
? | |
peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | requirement for O2 | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Rattus norvegicus |
7 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | about 70% of activity maximum at pH 6.0 and 7.5 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on | Rattus norvegicus | |
FAD | slight activity with | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
endorphin(51-61)NH2 | - |
Rattus norvegicus | |
0.001 | - |
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) | - |
Rattus norvegicus | |
0.005 | - |
adrenocorticotrophic hormone (1-10) | - |
Rattus norvegicus |