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Literature summary for 1.14.17.3 extracted from
- Identification, purification, and characterization of the molecular forms of Aplysia californica peptidylglycine alpha-amidating enzyme (1996), J. Neurochem., 66, 2596-2605.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | - |
Aplysia californica |  |
| Zn2+ | - |
Aplysia californica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | soluble form | Aplysia californica | 5737 | - |
| membrane | particulate form, except for abdominal ganglia | Aplysia californica | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 2fold increase in activity | Aplysia californica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
1 * 50000, membrane-bound form, SDS-PAGE | Aplysia californica |
| 60000 | - |
gel filtration | Aplysia californica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidylglycine + ascorbate + O2 | Aplysia californica | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aplysia californica | - |
marine mollusc | - |
| Aplysia californica | - |
2 forms: a soluble and a membrane-bound | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membrane-bound form from atrial gland | Aplysia californica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| atrial gland | - |
Aplysia californica | - |
| ganglion | abdominal and pleuropedal | Aplysia californica | - |
| heart | - |
Aplysia californica | - |
| additional information | tissue distribution, overview | Aplysia californica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aplysia californica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Tyr-Val-Gly + ascorbate + O2 | synthetic peptid | Aplysia californica | D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | - |
Aplysia californica | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | requirement for O2 | Aplysia californica | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Aplysia californica | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, membrane-bound form, SDS-PAGE | Aplysia californica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aplysia californica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Aplysia californica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | dependent on | Aplysia californica | |
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