KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
N-Dan-Tyr-Val-Gly | PHM activity in the soluble fraction of control and intermittent hypoxia brain stem ectracts | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
PHM, proteolytic processed, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
42000 | - |
PHM, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | PAM is the rate-limiting enzyme for the generation of carboxy-terminal alpha-amidated neuropeptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain stem | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000000112 | - |
PHM activity, normoxia, medulla, dorsal | Rattus norvegicus |
0.000000155 | - |
PAM activity, normoxia, control | Rattus norvegicus |
0.0000002 | - |
PHM activity, normoxia, medulla, ventral | Rattus norvegicus |
0.000000202 | - |
PHM activity, normoxia, control | Rattus norvegicus |
0.000000207 | - |
PAM activity, continuous hypobaric hypoxia, 10 days | Rattus norvegicus |
0.000000222 | - |
PHM activity, normoxia, pons, ventral | Rattus norvegicus |
0.000000268 | - |
PAM activity, intermittent hypoxia, 1 day | Rattus norvegicus |
0.000000298 | - |
PHM activity, intermittent hypoxia, 1 day | Rattus norvegicus |
0.000000312 | - |
PHM activity, intermittent hypoxia, medulla, dorsal | Rattus norvegicus |
0.000000315 | - |
PHM activity, continuous hypobaric hypoxia, 10 days | Rattus norvegicus |
0.000000578 | - |
PHM activity, intermittent hypoxia, 3 days | Rattus norvegicus |
0.000000648 | - |
PHM activity, intermittent hypoxia, 10 days | Rattus norvegicus |
0.00000066 | - |
PAM activity, intermittent hypoxia, 3 days | Rattus norvegicus |
0.000000667 | - |
PHM activity, normoxia, pons, dorsal | Rattus norvegicus |
0.0000008 | - |
PHM activity, intermittent hypoxia, medulla, ventral | Rattus norvegicus |
0.000000888 | - |
PHM activity, intermittent hypoxia, ventral | Rattus norvegicus |
0.000000907 | - |
PAM activity, intermittent hypoxia, 10 days | Rattus norvegicus |
0.0000016 | - |
PHM activity, intermittent hypoxia, pons, dorsal | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PAM is the rate-limiting enzyme for the generation of carboxy-terminal alpha-amidated neuropeptides | Rattus norvegicus | ? | - |
? | |
N-Dan-Tyr-Val-Gly + ascorbate + O2 | PAM activity assay, the product is unstable and dismutates to N-Dan-Tyr-Val-NH2 and glyoxylate (EC4.3.2.5) | Rattus norvegicus | N-Dan-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAM | PAM consists of O2-sensitive PHM and peptidyl-alpha-hydroxyglycine alpha-amidating lyase, PAL, activities | Rattus norvegicus |
peptidylglycine alpha-amidating monooxygenase | - |
Rattus norvegicus |
peptidylglycine alpha-hydroxylating monooxygenase | - |
Rattus norvegicus |
PHM | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
activity assay | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Rattus norvegicus |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | intermittent hypoxia activates peptidylglycine alpha-amidating monooxygenase via reactive oxygen species-mediated proteolytic processing | up |