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Literature summary for 1.14.18.1 extracted from
- Properties of phenoloxidase isolated from the cephalothorax of kuruma prawn (Penaeus japonicus). (2005), J. Food Biochem., 29, 470-485.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| methanol | the enzyme has an inactive proenzyme form which is activated by SDS and methanol, but not by trypsin, the partially purified active enzyme is not affected by copper acetate, SDS, methanol, and trypsin | Penaeus japonicus |  |
| SDS | the enzyme has an inactive proenzyme form which is activated by SDS and methanol, but not by trypsin, the partially purified active enzyme is not affected by copper acetate, SDS, methanol, and trypsin | Penaeus japonicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | at 5-10 mM | Penaeus japonicus | |
| L-cysteine | slight inhibition at 10 mM | Penaeus japonicus | |
| Phenylthiourea | - |
Penaeus japonicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a copper-containing enzyme | Penaeus japonicus | |
| additional information | the partially purified active enzyme is not affected by copper acetate, SDS, methanol, and trypsin | Penaeus japonicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
native PAGE, staining with L-beta-(3,4-dihydroxylphenyl)alanine | Penaeus japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penaeus japonicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native active enzyme partially, 19.4fold from cephalothorax, by ammonium sulfate fractionation and acetone precipitation | Penaeus japonicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cephalothorax | in the carapace, in the caudal zone and in the cuticle of the abdomen, mainly where the cuticle segments are joined and where the cuticle is connected to the pleopods | Penaeus japonicus | - |
| hemocyte | - |
Penaeus japonicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-DOPA + O2 | - |
Penaeus japonicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phenoloxidase | - |
Penaeus japonicus |
| PO | - |
Penaeus japonicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Penaeus japonicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity range | Penaeus japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, partially purified enzyme, completely stable | Penaeus japonicus |
| 50 | - |
30 min, partially purified enzyme, slight loss in activity | Penaeus japonicus |
| 60 | - |
30 min, partially purified enzyme, 22% remaining activity | Penaeus japonicus |
| 70 | 80 | 30 min, partially purified enzyme, complete inactivation | Penaeus japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Penaeus japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range | Penaeus japonicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | partially purified enzyme, stable, 90% of maximal activity within this range | Penaeus japonicus |
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