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Literature summary for 1.14.18.1 extracted from
- Tyrosinase activity in ionic liquids (2008), Biotechnol. Lett., 30, 153-158.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
4-methylcatechol | in aqueous solution | Agaricus bisporus |  |
| 0.5 | - |
4-methylcatechol | in CHCl3 | Agaricus bisporus | |
| 33 | - |
4-methylcatechol | in 1-butyl-3-methylimidazolium hexafluorophosphate, much higher than those obtained in CHCl3 and aqueous solution | Agaricus bisporus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme is extracted into 100 ml phosphate buffer (50 mM, pH 6.0) and crudely purified from fresh mushrooms (50 g) and then adsorbed onto celite (2 g) and dried in a vacuum oven | Agaricus bisporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of mushroom tyrosinase is studied in three ionic liquids, 1-butyl-3-methylimidazolium hexafluorophosphate (1-butyl-3-methylimidazolium hexafluorophosphate), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]) and 1-butyl-3-methylimidazolium methylsulfate ([BMIm] [MeSO4]), and is compared to that in chloroform. Enzyme follows in ionic liquids basically the same catalytic mechanism as in water | Agaricus bisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylcatechol + 1/2 O2 | oxidation of the substrate with NaIO4 in CHCl3, and [P]CHCl3 | Agaricus bisporus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyphenol oxidase | - |
Agaricus bisporus |
| tyrosinase | - |
Agaricus bisporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
optimal reaction temperature for the enzyme in chloroform | Agaricus bisporus |
| 45 | - |
optimal reaction temperature for the enzyme in aqueous solution | Agaricus bisporus |
| 50 | - |
optimal reaction temperature of the enzyme in 1-butyl-3-methylimidazolium hexafluorophosphate should be higher than 50°C, which is higher than the one for the enzyme in chloroform (20°C) and in aqueous solution (45°C) | Agaricus bisporus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Agaricus bisporus |
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Release 2023.1 (January 2023)
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