Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mimosine | inhibits monophenolhydroxylase and diphenoloxidase activity, inhibits the activity at concentrations in the microM-range, competitive inhibition | Palinurus elephas | |
Phenylthiourea | PTU, copper-chelating reagent, inhibits monophenolhydroxylase and diphenoloxidase activity, inhibits the activity strongly even at concentrations in the nM-range, noncompetitive inhibitor, inhibition with PTU is reversible | Palinurus elephas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
tyramine | inhibitor is mimosine | Palinurus elephas | |
1.6 | - |
tyramine | inhibitor is phenylthiourea | Palinurus elephas | |
3.4 | - |
dopamine | inhibitor is phenylthiourea | Palinurus elephas | |
4.2 | - |
dopamine | inhibitor is mimosine | Palinurus elephas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Palinurus elephas | - |
european spiny lobsters from the Northern Atlantic Sea | - |
Purification (Comment) | Organism |
---|---|
by ion exchange chromatography at pH 8.5 with a linear NaCl gradient | Palinurus elephas |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tyrosine + O2 = dopaquinone + H2O | EC 1.14.18.1, catalyze hydroxylation of monophenols to o-diphenols and their subsequent oxidation to o-quinones | Palinurus elephas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemolymph | obtained by punction through the intersegmental skin between the carapax and abdomen of a living animal | Palinurus elephas | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopamine + 1/2 O2 | diphenol | Palinurus elephas | dopamine quinone + H2O | - |
? | |
tyramine + O2 | monophenol | Palinurus elephas | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | detected by alkaline PAGE | Palinurus elephas |
Synonyms | Comment | Organism |
---|---|---|
tyrosinase | - |
Palinurus elephas |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
tyramine | inhibitor is mimosine | Palinurus elephas | |
5 | - |
tyramine | inhibitor is phenylthiourea | Palinurus elephas | |
135 | - |
dopamine | inhibitor is phenylthiourea | Palinurus elephas | |
144 | - |
dopamine | inhibitor is mimosine | Palinurus elephas |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000068 | - |
Phenylthiourea | with dopamine as substrate | Palinurus elephas | |
0.000892 | - |
Phenylthiourea | with tyramine as substrate | Palinurus elephas | |
0.172 | - |
mimosine | with tyramine as substrate | Palinurus elephas | |
0.536 | - |
mimosine | with dopamine as substrate | Palinurus elephas |