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Literature summary for 1.14.18.1 extracted from

  • Brack, A.; Hellmann, N.; Decker, H.
    Kinetic properties of hexameric tyrosinase from the crustacean Palinurus elephas (2008), Photochem. Photobiol., 84, 692-699.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
mimosine inhibits monophenolhydroxylase and diphenoloxidase activity, inhibits the activity at concentrations in the microM-range, competitive inhibition Palinurus elephas
Phenylthiourea PTU, copper-chelating reagent, inhibits monophenolhydroxylase and diphenoloxidase activity, inhibits the activity strongly even at concentrations in the nM-range, noncompetitive inhibitor, inhibition with PTU is reversible Palinurus elephas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
tyramine inhibitor is mimosine Palinurus elephas
1.6
-
tyramine inhibitor is phenylthiourea Palinurus elephas
3.4
-
dopamine inhibitor is phenylthiourea Palinurus elephas
4.2
-
dopamine inhibitor is mimosine Palinurus elephas

Organism

Organism UniProt Comment Textmining
Palinurus elephas
-
european spiny lobsters from the Northern Atlantic Sea
-

Purification (Commentary)

Purification (Comment) Organism
by ion exchange chromatography at pH 8.5 with a linear NaCl gradient Palinurus elephas

Reaction

Reaction Comment Organism Reaction ID
L-tyrosine + O2 = dopaquinone + H2O EC 1.14.18.1, catalyze hydroxylation of monophenols to o-diphenols and their subsequent oxidation to o-quinones Palinurus elephas

Source Tissue

Source Tissue Comment Organism Textmining
hemolymph obtained by punction through the intersegmental skin between the carapax and abdomen of a living animal Palinurus elephas
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dopamine + 1/2 O2 diphenol Palinurus elephas dopamine quinone + H2O
-
?
tyramine + O2 monophenol Palinurus elephas 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
?

Subunits

Subunits Comment Organism
hexamer detected by alkaline PAGE Palinurus elephas

Synonyms

Synonyms Comment Organism
tyrosinase
-
Palinurus elephas

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
tyramine inhibitor is mimosine Palinurus elephas
5
-
tyramine inhibitor is phenylthiourea Palinurus elephas
135
-
dopamine inhibitor is phenylthiourea Palinurus elephas
144
-
dopamine inhibitor is mimosine Palinurus elephas

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000068
-
Phenylthiourea with dopamine as substrate Palinurus elephas
0.000892
-
Phenylthiourea with tyramine as substrate Palinurus elephas
0.172
-
mimosine with tyramine as substrate Palinurus elephas
0.536
-
mimosine with dopamine as substrate Palinurus elephas