Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | effectively activates the enzyme activity at 0.1 mM | Aeromonas media | |
Triton X-100 | effectively activates the enzyme activity at 0.5 mM | Aeromonas media |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Aeromonas media |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arbutin | exhibits little inhibitory effect on TyrA with 25.6% inhibition at 10 mM | Aeromonas media | |
EDTA | highly effective inhibitor, complete inhibition at 1 mM | Aeromonas media | |
glutathione | highly effective inhibitor, complete inhibition at 0.5 mM | Aeromonas media | |
NaCl | 46.5% inhibition at 0.4 M | Aeromonas media | |
Phenylthiourea | 72.2% inhibition at 0.34 mM | Aeromonas media | |
tropolone | highly effective inhibitor, 94% inhibition at 10 mM | Aeromonas media |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
L-Dopa | endogenous enzyme, in 50 mM Tris-HCl (pH 7.0), at 30°C | Aeromonas media | |
1.16 | - |
L-Dopa | recombinant enzyme, in 50 mM Tris-HCl (pH 7.0), at 30°C | Aeromonas media |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE | Aeromonas media |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas media | B2Z3P7 | - |
- |
Aeromonas media WS | B2Z3P7 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography | Aeromonas media |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
enzyme from cell lysate, at 30°C | Aeromonas media |
34 | - |
purified enzyme, at 30°C | Aeromonas media |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + L-dopa + O2 | TyrA has a relatively higher affinity to L-DOPA than many other tyrosinases, the monophenol oxidase activity of this enzyme is undetectable when the concentration of L-dopa was lower than 0.01 mM, while the maximum monophenol oxidase activity is detected with 0.05 mM L-DOPA as cosubstrate | Aeromonas media | L-dopa + dopaquinone + H2O | - |
? | |
L-tyrosine + L-dopa + O2 | TyrA has a relatively higher affinity to L-DOPA than many other tyrosinases, the monophenol oxidase activity of this enzyme is undetectable when the concentration of L-dopa was lower than 0.01 mM, while the maximum monophenol oxidase activity is detected with 0.05 mM L-DOPA as cosubstrate | Aeromonas media WS | L-dopa + dopaquinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monophenol, o-diphenol: oxygen oxidoreductase | - |
Aeromonas media |
tyrA | has both monophenol oxidase and diphenol oxidase activity | Aeromonas media |
tyrosinase | - |
Aeromonas media |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
endogenous enzyme | Aeromonas media |
70 | - |
recombinant enzyme | Aeromonas media |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | endogenous TyrA is stable up to 50°C, but at higher temperatures, it starts to lose activity rapidly, shows half-life times of 30 days, 3 days, 2 days, 5 min, and 1 min at 30°C, 40°C, 50°C, 60°C, and 70°C, respectively. The activity of the recombinant enzyme declines rapidly as the temperature increases to above 40°C. | Aeromonas media |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the optimum pH for monophenol oxidase activity of endogenous and recombinant TyrA is determined to be pH 8.0 | Aeromonas media |
11 | - |
the optimum pH for diphenol oxidase activity of endogenous TyrA is determined to be pH 11.0 and pH 8.0 for recombinant TyrA | Aeromonas media |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | TyrA retains more than 90% of its activity after incubation at 4°C for 24 h in a pH range of 4.0-10.0 | Aeromonas media |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | TyrA exhibits optimal monophenol and diphenol oxidase activities under basic conditions (pH above 8.0), when the pH is higher than 10.0 the enzyme starts to lose its activity quickly, it loses nearly 50% of activity after 12 h at pH 11.0. Within the pH range of 4.0-10.0, unlike the endogenous enzyme, the recombinant enzyme shows good stability from neutral to moderately alkaline pH, while it is unstable at acidic conditions. | Aeromonas media |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aeromonas media | isoelectric focusing | - |
4.9 |
General Information | Comment | Organism |
---|---|---|
physiological function | key enzyme involved in melanin formation | Aeromonas media |