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Literature summary for 1.14.18.1 extracted from
- Purification and comparative characterization of monophenolase and diphenolase activities from a wild edible mushroom (Macrolepiota gracilenta) (2012), Process Biochem., 47, 2449-2454.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | - |
Macrolepiota gracilenta |  |
| benzoic acid | - |
Macrolepiota gracilenta | |
| Hg2+ | Hg2+ causes an inhibition in the range of 13% and 72% at 1 and 10 mM, respectively | Macrolepiota gracilenta | |
| L-cysteine | - |
Macrolepiota gracilenta | |
| L-phenylalanine | - |
Macrolepiota gracilenta | |
| Ni2+ | 87% inhibition at 10 mM | Macrolepiota gracilenta | |
| Sodium azide | - |
Macrolepiota gracilenta | |
| Sodium metabisulfite | - |
Macrolepiota gracilenta | |
| Thiourea | - |
Macrolepiota gracilenta | |
| Zn2+ | 82% inhibition at 10 mM | Macrolepiota gracilenta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
3,4-dihydroxyhydrocinnamic acid | at pH 7.0 and 22°C | Macrolepiota gracilenta | |
| 1 | - |
4-hydroxyphenylpropionic acid | at pH 7.0 and 22°C | Macrolepiota gracilenta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Macrolepiota gracilenta | |
| Mn2+ | - |
Macrolepiota gracilenta | |
| Ni2+ | - |
Macrolepiota gracilenta | |
| Zn2+ | - |
Macrolepiota gracilenta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57300 | - |
x * 57300, estimated from SDS-PAGE | Macrolepiota gracilenta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-DOPA + O2 | Macrolepiota gracilenta | 1% activity compared to 3,4-dihydroxyhydrocinnamic acid | dopaquinone + H2O | - |
? | |
| L-tyrosine + O2 | Macrolepiota gracilenta | 6% activity compared to 3,4-dihydroxyhydrocinnamic acid | dopaquinone + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macrolepiota gracilenta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone precipitation, Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column chromatography | Macrolepiota gracilenta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0402 | - |
crude extract, at pH 7.0 and 22°C | Macrolepiota gracilenta |
| 2.625 | - |
after 65.3fold purification, at pH 7.0 and 22°C | Macrolepiota gracilenta |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 5.0/7.0, 5 days, monophenolase/diphenolase activities of the enzyme are conserved approximately 40% and 60%, respectively | Macrolepiota gracilenta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxyhydrocinnamic acid + O2 | 100% activity | Macrolepiota gracilenta | ? | - |
? | |
| 4-hydroxyphenylpropionic acid + O2 | 85% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | ? | - |
? | |
| 4-methylcatechol + O2 | 90% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | ? | - |
? | |
| catechol + O2 | 12% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | ? | - |
? | |
| L-DOPA + O2 | 1% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | dopaquinone + H2O | - |
? | |
| L-tyrosine + O2 | 6% activity compared to 3,4-dihydroxyhydrocinnamic acid | Macrolepiota gracilenta | dopaquinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57300, estimated from SDS-PAGE | Macrolepiota gracilenta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cresolase | - |
Macrolepiota gracilenta |
| monophenol monooxygenase | - |
Macrolepiota gracilenta |
| polyphenol oxidase | - |
Macrolepiota gracilenta |
| PPO | - |
Macrolepiota gracilenta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
temperature optimum for diphenolase activity | Macrolepiota gracilenta |
| 40 | - |
temperature optimum for monophenolase activity | Macrolepiota gracilenta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | the diphenolase activity is more sensitive to temperatures above 40°C. Monophenolase activity conserves approximately 85% at 50°C. Diphenolase activity maintains its original activity 70% at 30°C at pH 5.0 after 1 day of incubation. The enzyme, for both activities, is completely inactivated at 60°C after 4 h | Macrolepiota gracilenta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
pH optimum for diphenolase activity | Macrolepiota gracilenta |
| 7 | - |
pH optimum for monophenolase activity | Macrolepiota gracilenta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
pH 7.0, the enzyme retains the diphenolase activity 80% at 4°C and 40% at 30°C. The enzyme loses diphenolase activity at all pH value sat 30°C after 5 day incubation | Macrolepiota gracilenta |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | Thiourea | |
| 0.03 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | ascorbic acid | |
| 0.06 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | Sodium metabisulfite | |
| 0.085 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | Thiourea | |
| 0.178 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | Sodium metabisulfite | |
| 0.24 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | L-cysteine | |
| 0.26 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | ascorbic acid | |
| 0.3 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | L-cysteine | |
| 0.5 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | Sodium azide | |
| 0.9 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | benzoic acid | |
| 3.56 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | L-phenylalanine | |
| 4.33 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | benzoic acid | |
| 13.8 | - |
diphenolase activity, at pH 5.0 and 30°C | Macrolepiota gracilenta | Sodium azide | |
| 16 | - |
monophenolase activity, at pH 7.0 and 40°C | Macrolepiota gracilenta | L-phenylalanine | |
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