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Literature summary for 1.14.18.1 extracted from

  • Palma-Orozco, G.; Marrufo-Hernandez, N.A.; Sampedro, J.G.; Najera, H.
    Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila) (2014), J. Agric. Food Chem., 62, 9832-9840 .
    View publication on PubMed
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid complete inhibition at 0.1 mM Mangifera indica
benzoic acid 31% inhibition at 0.1 mM, 52% inhibition at 10 mM Mangifera indica
Citric acid 30% inhibition at 0.1 mM, 33% inhibition at 10 mM Mangifera indica
CuSO4 87% inhibition at 0.1 mM, 50% inhibition at 10 mM Mangifera indica
EDTA 10% inhibition at 0.1 mM, 21% inhibition at 10 mM Mangifera indica
kojic acid 48% inhibition at 0.1 mM, complete inhibition at 10 mM Mangifera indica
MgCl2 10% inhibition at 0.1-1.0 mM, 11% inhibition at 10 mM Mangifera indica
NaCl 55% inhibition at 0.1 mM, 59% inhibition at 10 mM Mangifera indica
potassium sorbate 23% inhibition at 0.1 mM, 37% inhibition at 10 mM Mangifera indica
Sodium metabisulfite complete inhibition at 0.1 mM Mangifera indica
succinic acid 18% inhibition at 0.1 mM, 31% inhibition at 10 mM Mangifera indica
tropolone complete inhibition at 0.1 mM Mangifera indica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.77
-
 pyrogallol pH 7.0, 25°C Mangifera indica
3.14
-
 4-methylcatechol pH 7.0, 25°C Mangifera indica
15.14
-
 catechol pH 7.0, 25°C Mangifera indica

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 68% activation at 0.1 mM, 97% activation at 1 mM Mangifera indica
KCl 13% activation at 0.1 mM, 20% activation at 10 mM Mangifera indica
MgSO4 23% activation at 0.1 mM, 49% activation at 10 mM Mangifera indica
MnSO4 77% activation at 10 mM Mangifera indica
Na2SO4 95% activation at 10 mM Mangifera indica
ZnSO4 14% activation at 0.1 mM, 82% activation at 10 mM Mangifera indica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Mangifera indica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methylcatechol + O2 Mangifera indica
-
 ?
-
 ?
catechol + O2 Mangifera indica
-
 ?
-
 ?
pyrogallol + O2 Mangifera indica
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mangifera indica
-
 cv. Manila
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from fruit 216fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis, anion exchange chromatography, followed by gel filtration, to homogeneity Mangifera indica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Mangifera indica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8839
-
 purified native enzyme, pH 7.0, 25°C Mangifera indica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
 Mangifera indica ?
-
 ?
catechol + O2
-
 Mangifera indica ?
-
 ?
additional information caffeic acid, ferulic acid, epicatechin, and phenol are no substrates Mangifera indica ?
-
 ?
pyrogallol + O2
-
 Mangifera indica ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31500, SDS-PAGE Mangifera indica

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Mangifera indica
PPO
-
 Mangifera indica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Mangifera indica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, inactivation at 80°C Mangifera indica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 stable at Mangifera indica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Mangifera indica

pH Range

pH Minimum pH Maximum Comment Organism
3 7 activity range, drastic decrease in activity at pH 7.5 and above Mangifera indica