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Literature summary for 1.14.18.1 extracted from

  • Harir, M.; Bellahcene, M.; Baratto, M.C.; Pollini, S.; Rossolini, G.M.; Trabalzini, L.; Fatarella, E.; Pogni, R.
    Isolation and characterization of a novel tyrosinase produced by Sahara soil actinobacteria and immobilization on nylon nanofiber membranes (2017), J. Biotechnol., 265, 54-64 .
    View publication on PubMed
Search for more literature for 1.14.18.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons uncultured bacterium
DNA and amino acid sequence determination and analysis, sequence comparisons Streptomyces cyaneofuscatus

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilization on electrospun nylon nanofiber membranes uncultured bacterium
additional information enzyme immobilization on electrospun nylon nanofiber membranes Streptomyces cyaneofuscatus

Inhibitors

Inhibitors Comment Organism Structure
ascorbate complete inhibition at 0.1 mM Streptomyces cyaneofuscatus
EDTA 34% inhibition at 10 mM Streptomyces cyaneofuscatus
L-cysteine complete inhibition at 0.05 mM Streptomyces cyaneofuscatus
SDS 78% inhibition at 0.05 mM Streptomyces cyaneofuscatus
Sodium metabisulfite complete inhibition at 0.1 mM Streptomyces cyaneofuscatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces cyaneofuscatus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper-containing enzyme uncultured bacterium
Cu2+ a copper-containing enzyme Streptomyces cyaneofuscatus
additional information tyrosinase activity is enhanced in general by all the cations tested, apart for Ca2+ and Fe2+, that are poor inhibitors of the enzyme. No inhibition by 1 mM NaCl Streptomyces cyaneofuscatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-dopa + O2 uncultured bacterium
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces cyaneofuscatus
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces cyaneofuscatus Ms1
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces cyaneofuscatus NRRL B-2570
-
 2 dopaquinone + 2 H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone inhibits the activity 20-50% at 10-20% v/v Streptomyces cyaneofuscatus
Ethanol inhibits the activity 50% at 10% v/v Streptomyces cyaneofuscatus
Methanol inhibits the activity 50% at 30% v/v Streptomyces cyaneofuscatus

Organism

Organism UniProt Comment Textmining
Streptomyces cyaneofuscatus
-
 several strains are isolated from Algerian Sahara soil
-
Streptomyces cyaneofuscatus A0A2H4QH72
-
-
Streptomyces cyaneofuscatus Ms1
-
 several strains are isolated from Algerian Sahara soil
-
Streptomyces cyaneofuscatus NRRL B-2570 A0A2H4QH72
-
-
uncultured bacterium
-
 Sahara soil actinobacteria Ms1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 6.8fold by ammonium sulfate fractionation and anion exchange chromatography Streptomyces cyaneofuscatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.6
-
 purified enzyme, pH 7.0, temperature not specified in the publication Streptomyces cyaneofuscatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-dopa + O2
-
 uncultured bacterium 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces cyaneofuscatus 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces cyaneofuscatus Ms1 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces cyaneofuscatus NRRL B-2570 2 dopaquinone + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Streptomyces cyaneofuscatus

Synonyms

Synonyms Comment Organism
melC2
-
 Streptomyces cyaneofuscatus
MelC2 tyrosinase
-
 Streptomyces cyaneofuscatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 uncultured bacterium
55
-
-
 Streptomyces cyaneofuscatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 70 over 60% of maximal activity within this range uncultured bacterium
5 70 over 60% of maximal activity within this range Streptomyces cyaneofuscatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 purified enzyme, 90 min, stable at uncultured bacterium
30 40 purified enzyme, 90 min, stable at Streptomyces cyaneofuscatus
50
-
 purified enzyme, loss of 50% activity after 30 min, and of 80% activity after 90 min uncultured bacterium
50
-
 purified enzyme, loss of 50% activity after 30 min, and of 80% activity after 90 min Streptomyces cyaneofuscatus
60
-
 purified enzyme, inactivation within a few minutes uncultured bacterium
60
-
 purified enzyme, inactivation within a few minutes Streptomyces cyaneofuscatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at uncultured bacterium
7
-
 assay at Streptomyces cyaneofuscatus

General Information

General Information Comment Organism
evolution Ms1 genome scaffolds with publicly available Streptomyces genus whole genome sequences show the highest nucleotide identity (99%) with Streptomyces cyaneofuscatus strain NRRL B-2570, genes melC1 and melC2 uncultured bacterium