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Literature summary for 1.14.18.1 extracted from

  • Le Roes-Hill, M.; Palmer, Z.; Rohland, J.; Kirby, B.; Burton, S.
    Partial purification and characterisation of two actinomycete tyrosinases and their application in cross-linking reactions (2015), J. Mol. Catal. B, 122, 353-364 .
No PubMed abstract available
Search for more literature for 1.14.18.1

Application

Application Comment Organism
additional information when applied in various cross-linking reactions the tyrosinase is able to cross-link casein and gelatin in the absence of a phenolic compound, showing potential for application in the food industry and for the production of biomaterials Streptomyces pharetrae
additional information when applied in various cross-linking reactions the tyrosinase is able to cross-link casein and gelatine in the absence of a phenolic compound, showing potential forapplication in the food industry and for the production of biomaterials Streptomyces polyantibioticus

Inhibitors

Inhibitors Comment Organism Structure
arbutin
-
 Streptomyces pharetrae
arbutin
-
 Streptomyces polyantibioticus
Co2+ 22% inhibition at 5 mM Streptomyces polyantibioticus
Cu2+ over 60% inhibition at 5 mM Streptomyces pharetrae
Cu2+ over 60% inhibition at 5 mM Streptomyces polyantibioticus
EDTA
-
 Streptomyces pharetrae
EDTA
-
 Streptomyces polyantibioticus
Fe2+ complete inhibition at 5 mM Streptomyces pharetrae
additional information the enzyme is strongly inhibited by the reducing agents Streptomyces pharetrae
additional information the enzyme is strongly inhibited by the reducing agents Streptomyces polyantibioticus
sodium chloride
-
 Streptomyces pharetrae
sodium chloride
-
 Streptomyces polyantibioticus
sodium dodecylsulfate
-
 Streptomyces pharetrae
sodium dodecylsulfate
-
 Streptomyces polyantibioticus
Zn2+ 70% inhibition at 5 mM Streptomyces pharetrae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at 5 mM Streptomyces pharetrae
Cu2+ a copper-containing enzyme Streptomyces pharetrae
Cu2+ a copper-containing enzyme Streptomyces polyantibioticus
Mg2+ activates at 5 mM Streptomyces pharetrae
Mn2+ activates at 5 mM Streptomyces pharetrae
additional information no effect by Co2+ at 5 mM Streptomyces pharetrae
additional information no effect by Zn2+ and Fe2+ at 5 mM Streptomyces polyantibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-dopa + O2 Streptomyces pharetrae
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces polyantibioticus
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces polyantibioticus SPR
-
 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2 Streptomyces pharetrae CZA14
-
 2 dopaquinone + 2 H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 10% activity remaining at 40% v/v, 0% at 50% v/v Streptomyces pharetrae
2-propanol 70% activity remaining at 30% v/v, below 5% at 40% v/v Streptomyces polyantibioticus
Acetone 20% activity remaining at 45% v/v, 0% at 50% v/v Streptomyces polyantibioticus
Acetone 75% activity remaining at 45% v/v, 18% at 50% v/v Streptomyces pharetrae
acetonitrile 10% activity remaining at 30% v/v, 0% at 40% v/v Streptomyces pharetrae
acetonitrile 70% activity remaining at 20% v/v, 0% at 30% v/v Streptomyces polyantibioticus
DMSO 80% activity remaining at 40% v/v, 65% at 50% v/v Streptomyces pharetrae
DMSO over 85% activity remaining at 40% v/v, 65% at 50% v/v Streptomyces polyantibioticus
Ethanol 65% activity remaining at 40% v/v, 15% at 50% v/v Streptomyces pharetrae
Ethanol 80% activity remaining at 30% v/v, 0% at 40% v/v Streptomyces polyantibioticus
Methanol 20% activity remaining at 40% v/v, 0% at 50% v/v Streptomyces polyantibioticus
Methanol 70% activity remaining at 40% v/v, 18% at 50% v/v Streptomyces pharetrae

Organism

Organism UniProt Comment Textmining
Streptomyces pharetrae
-
-
-
Streptomyces pharetrae CZA14
-
-
-
Streptomyces polyantibioticus
-
-
-
Streptomyces polyantibioticus SPR
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 11.84fold by ammonium sulfate fractionation, dialysis, and separation by additin of 20% (w/v) PEG 8000 solution, followed by anion exchange chromatography Streptomyces polyantibioticus
native enzyme 5.16fold by ammonium sulfate fractionation, dialysis, and separation by additin of 20% (w/v) PEG 8000 solution, followed by anion exchange chromatography Streptomyces pharetrae

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimizatin of tyrosinase production for industrial applications, method optimization, overview Streptomyces pharetrae
-
additional information optimizatin of tyrosinase production for industrial applications, method optimization, overview Streptomyces polyantibioticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.07
-
 purified enzyme, pH 6.5, 22°C Streptomyces pharetrae
23.8
-
 purified enzyme, pH 6.5, 22°C Streptomyces polyantibioticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-dopa + O2
-
 Streptomyces pharetrae 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces polyantibioticus 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces polyantibioticus SPR 2 dopaquinone + 2 H2O
-
 ?
2 L-dopa + O2
-
 Streptomyces pharetrae CZA14 2 dopaquinone + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 30500, SDS-PAGE Streptomyces polyantibioticus
? x * 32600, SDS-PAGE Streptomyces pharetrae

Synonyms

Synonyms Comment Organism
CZA14Tyr
-
 Streptomyces pharetrae
melC2
-
 Streptomyces pharetrae
melC2
-
 Streptomyces polyantibioticus
SPRTyr
-
 Streptomyces polyantibioticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 35 first optimum Streptomyces polyantibioticus
30 40 first optimum Streptomyces pharetrae
50
-
 second optimum Streptomyces polyantibioticus
60
-
 second optimum Streptomyces pharetrae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 enzyme CZA14Tyr, 1 h, stable up to Streptomyces pharetrae
40
-
 enzyme SPRTyr , 1 h, stable up to Streptomyces polyantibioticus
60
-
 enzyme CZA14Tyr shows residual activity of 29.50% (0.53% for SPRTyr) after 10 min incubation at 60°C, but rapidly loses 97% activity after 20 min of incubation Streptomyces pharetrae
60
-
 enzyme SPRTyr shows residual activity of 0.53% after10 min incubation at 60°C meaning inactivation Streptomyces polyantibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptomyces pharetrae
6.5
-
-
 Streptomyces polyantibioticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 enzyme SPRTyr, stable at Streptomyces polyantibioticus
6.5 10 enzyme CZA14Tyr, stable at Streptomyces pharetrae