Activating Compound | Comment | Organism | Structure |
---|---|---|---|
membrane-associated transporter protein | MATP, encoded by gene SLC45A2, regulates melanosomal pH and influences tyrosinase activity, mechanism modeling, overview. The SLC45A2 transcript is highly expressed in human melanoma cells and primary melanocyte. Knockdown of MATP using siRNAs reduces melanin content and tyrosinase activity without any morphological change in melanosomes or the expression of melanogenesis-related proteins, and lowers the the melanosomal pH. The reduced tyrosinase activity in MATP-knockdown cells is due to improper copper binding to tyrosinase under the acidic conditions in melanosomes and that the acidic conditions are due to MATP deletion | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
melanosome | - |
Homo sapiens | 42470 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper-containing enzyme, copper is an important element for tyrosinase activity, binding to tyrosinase depends on pH in melanosomes | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | Homo sapiens | - |
2 dopaquinone + 2 H2O | - |
? | |
tyrosine + O2 | Homo sapiens | - |
dopaquinone + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation is an important factor for tyrosinase activity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | - |
Homo sapiens | - |
hair | - |
Homo sapiens | - |
melanocyte | primary | Homo sapiens | - |
melanoma cell | - |
Homo sapiens | - |
MNT-1 cell | - |
Homo sapiens | - |
skin | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-dopa + O2 | - |
Homo sapiens | 2 dopaquinone + 2 H2O | - |
? | |
tyrosine + O2 | - |
Homo sapiens | dopaquinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-DOPA oxidase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | the reduction of tyrosinase activity associated with the knockdown of membrane-associated transporter protein, MATP, using siRNA is readily recovered by copper treatment in the in vitro L-DOPA oxidase activity assay of tyrosinase. N-glycosylation is an important factor for tyrosinase activity, which was reduced in the MATP-knockdown cells | Homo sapiens |
physiological function | membrane-associated transporter protein, MATP, may play an important role in regulating tyrosinase activity via controlling melanosomal pH | Homo sapiens |