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Literature summary for 1.14.18.2 extracted from
- Isolation and characterization of cytidine-5'-monophosphate-N-acetylneuraminate hydroxylase from the starfish Asterias rubens (2003), Comp. Biochem. Physiol. B, 134, 89-101.
General Stability
| General Stability | Organism |
|---|---|
| after each cycle of freezing and thawing, 25% loss of activity | Asterias rubens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Asterias rubens |  |
| 2,2'-dipyridyl | - |
Asterias rubens | |
| ferrozine | - |
Asterias rubens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | maximal 3.5fold stimulation at 100 mM | Asterias rubens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Asterias rubens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Asterias rubens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gonad | - |
Asterias rubens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00093 | - |
- |
Asterias rubens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80 C, very stable | Asterias rubens |
| 4°C, 48 h, 10% loss of activity | Asterias rubens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 | evidence for the formation of a ternary complex of hydroxylase, CMP-Neu5Ac and cytochrome b5 | Asterias rubens | CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O | - |
? | |
| CMP-N-acetylneuraminate + NADH + O2 | - |
Asterias rubens | CMP-N-glycoloylneuraminate + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | a main protein of 76000 da and a minor protein of 64000 Da correlates with CMP-neu5Ac hydroxylase activity | Asterias rubens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
48 h, 10% loss of activity | Asterias rubens |
| 25 | - |
2 h, 50% loss of activity | Asterias rubens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ferrocytochrome b5 | evidence for the formation of a ternary complex of hydroxylase, CMP-Neu5Ac and cytochrome b5 | Asterias rubens | |
| NADH | - |
Asterias rubens | |
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Release 2023.1 (January 2023)
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