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Literature summary for 1.14.18.2 extracted from
- Production and purification of secretory simian cytidine monophosphate-N-acetylneuraminic acid hydroxylase using baculovirus-protein expression system (2015), Biol. Pharm. Bull., 38, 1220-1226 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme CMAH can be used for large-scale production of N-glycolylneuraminic acid | Chlorocebus aethiops |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional recombinant expression of secretory His-tagged enzyme, containing an N-terminal secretion signal peptide, in Spodoptera frugiperda Sf9 cells using the baculovirus infection method, large scale enzyme production | Chlorocebus aethiops |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ | Chlorocebus aethiops | - |
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorocebus aethiops | - |
- |
- |
| no activity in Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the secreted recombinant His-tagged enzyme contains N-glycans | Chlorocebus aethiops |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant secretory His-tagged enzyme from Spodoptera frugiperda Sf9 cell culture supernatant by affinity chromatography and ultrafiltration | Chlorocebus aethiops |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| COS-7 cell | - |
Chlorocebus aethiops | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Chlorocebus aethiops | CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x + 65000, secreted recombinant His-tagged enzyme, SDS-PAGE | Chlorocebus aethiops |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMAH | - |
Chlorocebus aethiops |
| CMP Neu5Ac hydroxylase | - |
Chlorocebus aethiops |
| cytidine monophosphate-N-acetylneuraminic acid hydroxylase | - |
Chlorocebus aethiops |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Chlorocebus aethiops |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Chlorocebus aethiops |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b5 | - |
Chlorocebus aethiops |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | sequence-based, three-dimensional enzyme structure modeling, overview | Chlorocebus aethiops |
| physiological function | the enzyme is essential for N-glycolylneuraminic acid (Neu5Gc) synthesis | Chlorocebus aethiops |
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Release 2023.1 (January 2023)
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