Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.18.3 extracted from

  • Shiemke, A.K.; Cook, S.A.; Miley, T.; Singleton, P.
    Detergent solubilization of membrane-bound methane monooxygenase requires plastoquinol analogs as electron donors (1995), Arch. Biochem. Biophys., 321, 421-428.
    View publication on PubMed
Search for more literature for 1.14.18.3

Activating Compound

Activating Compound Comment Organism Structure
lauryl maltoside the stimulatory effect of lauryl maltoside is responsible for the initial increase in duroquinol-dependent activity of the pellet, but no activity with NADH is observed after this solubilization Methylococcus capsulatus
additional information no pMMO acivity is observed in the detergent-solubilized fraction in the presence of dithionite, ascorbate, or methyl viologen. No pMMO activity with duroquinol or NADH is observed after solubilization with Triton X-100, Tween 20, zwittergent 3-12, Nonidet-P40, or synperonic Methylococcus capsulatus

Inhibitors

Inhibitors Comment Organism Structure
Acetylene 4.3% residual activity at 10% (v/v) Methylococcus capsulatus
cyanide less than 2% residual activity at 2 mM Methylococcus capsulatus
EDTA 18.1% residual activity at 1.5 mM Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methylococcus capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme is stimulated by exogenous copper (348% activity at 0.4 mM) Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus Bath
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H2O2
-
 Methylococcus capsulatus ?
-
 ?
Mn2+ + H2O2
-
 Methylococcus capsulatus Bath ?
-
 ?
additional information quinols are effective reductants for the detergent-solubilized enzyme, whereas NADH is ineffective Methylococcus capsulatus ?
-
 ?
additional information quinols are effective reductants for the detergent-solubilized enzyme, whereas NADH is ineffective Methylococcus capsulatus Bath ?
-
 ?
propylene + 2,3-dimethylquinol + O2
-
 Methylococcus capsulatus propylene oxide + 2,3-dimethylquinone + H2O
-
 ?
propylene + 2,3-dimethylquinol + O2
-
 Methylococcus capsulatus Bath propylene oxide + 2,3-dimethylquinone + H2O
-
 ?
propylene + coenzyme Q0 + O2
-
 Methylococcus capsulatus propylene oxide + reduced coenzyme Q0 + H2O
-
 ?
propylene + coenzyme Q0 + O2
-
 Methylococcus capsulatus Bath propylene oxide + reduced coenzyme Q0 + H2O
-
 ?
propylene + decyl-plastoquinol + O2 higher activity compared to duroquinol Methylococcus capsulatus propylene oxide + decyl-plastoquinone + H2O
-
 ?
propylene + decyl-plastoquinol + O2 higher activity compared to duroquinol Methylococcus capsulatus Bath propylene oxide + decyl-plastoquinone + H2O
-
 ?
propylene + decylubiquinol + O2
-
 Methylococcus capsulatus propylene oxide + decylubiquinone + H2O
-
 ?
propylene + duroquinol + O2
-
 Methylococcus capsulatus propylene oxide + duroquinone + H2O
-
 ?
propylene + menaquinol + O2
-
 Methylococcus capsulatus propylene oxide + menaquinone + H2O
-
 ?
propylene + trimethylquinol + O2
-
 Methylococcus capsulatus propylene oxide + trimethylquinone + H2O
-
 ?

Synonyms

Synonyms Comment Organism
pMMO
-
 Methylococcus capsulatus